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Team:Calgary/20 June 2009
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| - | + | PCR of luxPQ in psB1AK3 using BBK-CP-F/R and gene specific primers | |
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| - | + | Purpose: to verify presence of LuxPQ in psB1AK3. Colony 4 of luxPQ in psB1AK3 was used as a template and pTaq DNA polymerase was used. LuxPQ-F/R gene specific primers and BBK-CP-F/R primers (that anneal just outside the BBK prefix and suffix) were used. Conditions were as follows: 94ºC for 2 minutes; 36X (94ºC for 30 seconds; 55ºC for 45 seconds for BBK-CP-F/R primers (53ºC for 45 seconds for LuxPQ F/R primers); 72ºC for 4 minutes); 72ºC for 10 minutes; held at 4ºC. The products were then run on a 0.8% agarose gel (see below). | |
| - | + | [[Image:2009.06.22.LuxPQ_BBKCP_BBK.png|700px]] | |
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Revision as of 19:19, 2 September 2009
UNIVERSITY OF CALGARY
