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Template:Team:KULeuven/3 August 2009/VanillinProduction
From 2009.igem.org
(Difference between revisions)
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| - | We received the ComT, Sam5 and Sam8 from Edinburgh today. Unfortunately, the tube containing the Sam5 + RBS (with the PstI site) | + | We received the ComT, Sam5 and Sam8 from Edinburgh today. Unfortunately, the tube containing the Sam5 + RBS (with the PstI site) was opened during transport and all DNA in it was lost. The others were transferred into competent cells by electroporation. |
* Sam5 + RBS in {{kulpart|pSB1A2}} (with PstI site removed), {{kulpart|BBa_K238007}} | * Sam5 + RBS in {{kulpart|pSB1A2}} (with PstI site removed), {{kulpart|BBa_K238007}} | ||
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* ComT in {{kulpart|pSB1A2}} coding sequence, {{kulpart|BBa_I742107}} | * ComT in {{kulpart|pSB1A2}} coding sequence, {{kulpart|BBa_I742107}} | ||
| - | The cells were put in liquid LB to recuperate from the procedure | + | The cells were put in liquid LB to recuperate from the procedure, transferred onto LB/amp plates and grown overnight at 37°C. |
We also made liquid cultures of the RBS + fcs and RBS + ech cultures so that we can miniprep them tomorrow. | We also made liquid cultures of the RBS + fcs and RBS + ech cultures so that we can miniprep them tomorrow. | ||
Revision as of 08:12, 8 September 2009
We received the ComT, Sam5 and Sam8 from Edinburgh today. Unfortunately, the tube containing the Sam5 + RBS (with the PstI site) was opened during transport and all DNA in it was lost. The others were transferred into competent cells by electroporation.
- Sam5 + RBS in (with PstI site removed),
- Sam8 + RBS in ,
- Sam8 in coding sequence,
- ComT in coding sequence,
The cells were put in liquid LB to recuperate from the procedure, transferred onto LB/amp plates and grown overnight at 37°C.
We also made liquid cultures of the RBS + fcs and RBS + ech cultures so that we can miniprep them tomorrow.
