Template:Team:KULeuven/7 September 2009/VanillinProduction

From 2009.igem.org

(Difference between revisions)
(New page: * Miniprep from the four colonies of EF ligation ** Accidentally used double volume of elution buffer on EF4 {| border ="1" align="center" !| part || concentration || 260/280 || 260/230 |-...)
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|- align="center"
|- align="center"
| EF 4 || 52,7 || 2,06 || 0,97
| EF 4 || 52,7 || 2,06 || 0,97
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|}
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* Gel electrophorese of Restriction from Sam5 and Sam8, looked good
 +
* Purified and ligated the restriction from Sam5 and Sam8 overnight
 +
** Concentration for sam8 was barely enough for ligation...
 +
{| border ="1" align="center"
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!| part || concentration || 260/280 || 260/230
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|- align="center"
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| Sam5 || 10,1 || 2,11 || 0,04
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|- align="center"
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| Sam8 || 3,1 || 3,96 || 0,02
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|-
|}
|}

Revision as of 15:13, 8 September 2009

  • Miniprep from the four colonies of EF ligation
    • Accidentally used double volume of elution buffer on EF4
part concentration 260/280 260/230
EF 1 115,5 1,98 2,31
EF 2 105,2 1,96 1,66
EF 3 95,1 12,05 0,98
EF 4 52,7 2,06 0,97
  • Gel electrophorese of Restriction from Sam5 and Sam8, looked good
  • Purified and ligated the restriction from Sam5 and Sam8 overnight
    • Concentration for sam8 was barely enough for ligation...
part concentration 260/280 260/230
Sam5 10,1 2,11 0,04
Sam8 3,1 3,96 0,02
Retrieved from "http://2009.igem.org/Template:Team:KULeuven/7_September_2009/VanillinProduction"