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Team:KULeuven/22 July 2009
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| - | {{Team:KULeuven/ | + | {{Team:KULeuven/Common2/BeginHeader}} |
| - | {{Team:KULeuven/Common/ | + | {{Team:KULeuven/Common/SubMenu_Notebook}} |
| - | {{Team:KULeuven/ | + | {{Team:KULeuven/Common2/EndHeader}} |
{{Team:KULeuven/Notebook/DayNavigator}} | {{Team:KULeuven/Notebook/DayNavigator}} | ||
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==Wet Lab== | ==Wet Lab== | ||
| + | * Grown parts in liquid medium | ||
| + | ** {{kulpart|BBa_B0015}} | ||
| + | ** {{kulpart|BBa_B0032}} | ||
| + | ** {{kulpart|BBa_J23110}} | ||
| + | ** {{kulpart|BBa_J23078}} | ||
| + | |||
| + | ==Dry Lab== | ||
| + | |||
| + | Over the last few days the blue light sensor, and the vanillin production | ||
| + | were modeled in simbiology from mathworks. | ||
| + | Most of the kinetic parameters are unknown. | ||
== Project == | == Project == | ||
| - | The Vanillin Receptor is not usable, Madrid also tried | + | The Vanillin Receptor is not usable, Madrid also tried and ran into all sorts of problems: |
''Problems span from the binding of the periplasmic protein to the vanillin molecule, to the function of the fusion protein, to the activation of the ompR.'' | ''Problems span from the binding of the periplasmic protein to the vanillin molecule, to the function of the fusion protein, to the activation of the ompR.'' | ||
| + | |||
There are two solutions to this problem: | There are two solutions to this problem: | ||
# Construct a different vanillin receptor | # Construct a different vanillin receptor | ||
| Line 16: | Line 28: | ||
# Detect an alternative substance that has a linear relationship with the vanillin concentration | # Detect an alternative substance that has a linear relationship with the vanillin concentration | ||
#* Vanillin inhibits the Ahl receptor | #* Vanillin inhibits the Ahl receptor | ||
| - | #* Synthesis of another signaling molecule that | + | #* Synthesis of another signaling molecule that could be sensed |
| + | {{Team:KULeuven/Common2/PageFooter}} | ||
Latest revision as of 08:39, 10 September 2009
Contents |
Project progress
Wet Lab
- Grown parts in liquid medium
Dry Lab
Over the last few days the blue light sensor, and the vanillin production were modeled in simbiology from mathworks. Most of the kinetic parameters are unknown.
Project
The Vanillin Receptor is not usable, Madrid also tried and ran into all sorts of problems: Problems span from the binding of the periplasmic protein to the vanillin molecule, to the function of the fusion protein, to the activation of the ompR.
There are two solutions to this problem:
- Construct a different vanillin receptor
- Using VirA/VirG, a phenol receptor in agrobacterium .
- Detect an alternative substance that has a linear relationship with the vanillin concentration
- Vanillin inhibits the Ahl receptor
- Synthesis of another signaling molecule that could be sensed
