"
Template:Team:KULeuven/7 September 2009/VanillinProduction
From 2009.igem.org
(Difference between revisions)
JochemDeen (Talk | contribs) |
|||
| Line 1: | Line 1: | ||
* Miniprep from the four colonies of EF ligation | * Miniprep from the four colonies of EF ligation | ||
| - | ** Accidentally used double | + | ** Accidentally used double volumes of elution buffer on EF4 |
{| border ="1" align="center" | {| border ="1" align="center" | ||
!| part || concentration || 260/280 || 260/230 | !| part || concentration || 260/280 || 260/230 | ||
| Line 13: | Line 13: | ||
|- | |- | ||
|} | |} | ||
| - | * Gel | + | * Gel electrophoresis of restriction from Sam5 and Sam8 looked good |
* Purified and ligated the restriction from Sam5 and Sam8 overnight | * Purified and ligated the restriction from Sam5 and Sam8 overnight | ||
** Concentration for sam8 was barely enough for ligation... | ** Concentration for sam8 was barely enough for ligation... | ||
Revision as of 10:07, 10 September 2009
- Miniprep from the four colonies of EF ligation
- Accidentally used double volumes of elution buffer on EF4
| part | concentration | 260/280 | 260/230 |
|---|---|---|---|
| EF 1 | 115,5 | 1,98 | 2,31 |
| EF 2 | 105,2 | 1,96 | 1,66 |
| EF 3 | 95,1 | 12,05 | 0,98 |
| EF 4 | 52,7 | 2,06 | 0,97 |
- Gel electrophoresis of restriction from Sam5 and Sam8 looked good
- Purified and ligated the restriction from Sam5 and Sam8 overnight
- Concentration for sam8 was barely enough for ligation...
| part | concentration | 260/280 | 260/230 |
|---|---|---|---|
| Sam5 | 10,1 | 2,11 | 0,04 |
| Sam8 | 3,1 | 3,96 | 0,02 |
