"
Team:Imperial College London/Wetlab/Protocols
From 2009.igem.org
(Difference between revisions)
(→Autoinduction assays) |
|||
| Line 2: | Line 2: | ||
=Autoinduction assays= | =Autoinduction assays= | ||
| - | [[Team:Imperial_College_London/Wetlab/Protocols/Glucose delay|Glucose time delay]]<br> | + | ==Glucose time delay== |
| + | ===Aims=== | ||
| + | |||
| + | * Characterise the tunable time duration it takes before GFP expression (M2 activation) | ||
| + | |||
| + | ===Assay=== | ||
| + | |||
| + | *The cells will be grown until OD= 0.7. | ||
| + | |||
| + | *The RFP value will be monitered, although it is the GFP values that are more critical in this assay. <br> | ||
| + | |||
| + | *OD and fluorescence data for GFP which can be converted in [http://partsregistry.org/cgi/measurement/new_batch.cgi Specific Promoter Units (SPUs) by a calculator found on the Registry of Standard Biological Parts]. | ||
| + | <br> | ||
| + | |||
| + | *The secondary carbon source will be taken from the previous experiment (see Secondary carbon source selection for CRP promoter)<br> | ||
| + | |||
| + | *The IPTG concentrations will be taken from the previous experiment (see Determining concentration of IPTG) <br> | ||
| + | |||
| + | [[Team:Imperial_College_London/Wetlab/Protocols/Glucose delay|See Glucose time delay for details]]<br> | ||
| + | |||
=Protein production assays= | =Protein production assays= | ||
[[Team:Imperial_College_London/Wetlab/Protocols/Cellulase| Cellulase Assay]]<br> | [[Team:Imperial_College_London/Wetlab/Protocols/Cellulase| Cellulase Assay]]<br> | ||
Revision as of 13:48, 10 September 2009
Contents |
Autoinduction assays
Glucose time delay
Aims
- Characterise the tunable time duration it takes before GFP expression (M2 activation)
Assay
- The cells will be grown until OD= 0.7.
- The RFP value will be monitered, although it is the GFP values that are more critical in this assay.
- OD and fluorescence data for GFP which can be converted in [http://partsregistry.org/cgi/measurement/new_batch.cgi Specific Promoter Units (SPUs) by a calculator found on the Registry of Standard Biological Parts].
- The secondary carbon source will be taken from the previous experiment (see Secondary carbon source selection for CRP promoter)
- The IPTG concentrations will be taken from the previous experiment (see Determining concentration of IPTG)
See Glucose time delay for details
Protein production assays
Encapsulation assays
Genomic deletion assays
