Template:Team:KULeuven/6 September 2009/VanillinProduction
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** Transfered 4 colonies from EF ligation to a new plate | ** Transfered 4 colonies from EF ligation to a new plate | ||
** Put the 4 transfered colonies on liquid medium | ** Put the 4 transfered colonies on liquid medium | ||
+ | a miniprep and restrictie digest (ON) on sam5, sam8, ech and fcs was performed on saterday. on sunday, a gel was run to check the inserts. These were good so a motherplate for all the genes could be made. |
Revision as of 08:19, 11 September 2009
- Restriction digest overnight of Sam5 (1,2) and Sam8 (1,2)
- Growth of EF Ligation (woohoo!)
- Transfered 4 colonies from EF ligation to a new plate
- Put the 4 transfered colonies on liquid medium
a miniprep and restrictie digest (ON) on sam5, sam8, ech and fcs was performed on saterday. on sunday, a gel was run to check the inserts. These were good so a motherplate for all the genes could be made.