"
Template:Team:KULeuven/11 September 2009/BlueLightReceptor
From 2009.igem.org
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | # the electroporated ligY did not grow. A new restrictie digest on pcr product {{kulpart|BBa_K238013}} was performed with EcoRI and PstI. | + | # the electroporated ligY did not grow. A new restrictie digest on pcr product {{kulpart|BBa_K238013}} was performed with EcoRI and PstI.This was put on gel. there was a good signal so it was used to perform a ligation with vector pSB1A2. |
# a liquid culture that was made from pSB3K3 a few days ago showed growth after all. this was miniprepped and restrictie digested to check wether the vector is actually present. | # a liquid culture that was made from pSB3K3 a few days ago showed growth after all. this was miniprepped and restrictie digested to check wether the vector is actually present. | ||
| + | # the liquid cultures with our blue light construct (lig) that stood overnight at 16°C was FACS-ed. half of the cultures were exposed to blue light during the day and put under the FACS in the evening. | ||
Revision as of 14:03, 11 September 2009
- the electroporated ligY did not grow. A new restrictie digest on pcr product was performed with EcoRI and PstI.This was put on gel. there was a good signal so it was used to perform a ligation with vector pSB1A2.
- a liquid culture that was made from pSB3K3 a few days ago showed growth after all. this was miniprepped and restrictie digested to check wether the vector is actually present.
- the liquid cultures with our blue light construct (lig) that stood overnight at 16°C was FACS-ed. half of the cultures were exposed to blue light during the day and put under the FACS in the evening.
