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Team:Imperial College London/M1/Modelling
From 2009.igem.org
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| - | {{Imperial/Box1|Module 1|Two models are required to explain the functionality of M1: | + | =Introduction= |
| + | This module consists of producing the drug protein of interest. In order to describe the function of the module, two models have been developed. | ||
| + | |||
| + | ==Model for protein drug production:== | ||
| + | Based on the [https://2009.igem.org/Team:Imperial_College_London/M1/Genetic Genetic circuit], a LacI-IPTG inducible promoter is responsible for kickstarting the production of the drug. | ||
| + | * In the absence of IPTG, LacI represses the production of the drug (Cellulase or PAH) | ||
| + | * When IPTG is introduced, the LacI repressing pathway is “de-repressed”, and some output protein is produced. | ||
| + | [[Image:Genetic circuit.jpg]] | ||
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| + | ===Our goals=== | ||
| + | The modelling aims to provide an overview and better understanding of the M1 system’s function by: | ||
| + | *Characterizing the system. | ||
| + | *Modeling to account for several factors that may reduce/hinder the production of our output protein of interest such as: | ||
| + | **Lac promoter leakiness | ||
| + | **IPTG toxicity | ||
| + | **Stability of output protein | ||
| + | |||
| + | |||
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| + | {{Imperial/Box1|Module 1: Protein production|Two models are required to explain the functionality of M1: | ||
1) Modelling protein production - Integral part of our design, responsible for manufacturing the drug of interest. | 1) Modelling protein production - Integral part of our design, responsible for manufacturing the drug of interest. | ||
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This model will allow an understanding of the activity of the enzymes and allow the WETLAB to have an idea of the magnitude of the activity of the enzymes. More importantly, after the results from enzyme assays are obtained, the model should provide a means of relating the activity output data to the concentration of the enzyme. <br> | This model will allow an understanding of the activity of the enzymes and allow the WETLAB to have an idea of the magnitude of the activity of the enzymes. More importantly, after the results from enzyme assays are obtained, the model should provide a means of relating the activity output data to the concentration of the enzyme. <br> | ||
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Revision as of 12:10, 12 September 2009
Introduction
This module consists of producing the drug protein of interest. In order to describe the function of the module, two models have been developed.
Model for protein drug production:
Based on the Genetic circuit, a LacI-IPTG inducible promoter is responsible for kickstarting the production of the drug.
- In the absence of IPTG, LacI represses the production of the drug (Cellulase or PAH)
- When IPTG is introduced, the LacI repressing pathway is “de-repressed”, and some output protein is produced.
Our goals
The modelling aims to provide an overview and better understanding of the M1 system’s function by:
- Characterizing the system.
- Modeling to account for several factors that may reduce/hinder the production of our output protein of interest such as:
- Lac promoter leakiness
- IPTG toxicity
- Stability of output protein
