Introduction

This module consists of producing the drug protein of interest. In order to describe the function of the module, two models have been developed.

Model for protein drug production:

Based on the Genetic circuit, a LacI-IPTG inducible promoter is responsible for kickstarting the production of the drug.

• In the absence of IPTG, LacI represses the production of the drug (Cellulase or PAH)
• When IPTG is introduced, the LacI repressing pathway is “de-repressed”, and some output protein is produced.

Our goals

The modelling aims to provide an overview and better understanding of the M1 system’s function by:

• Characterizing the system.
• Modeling to account for several factors that may reduce/hinder the production of the protein drug such as:
  • Lac promoter leakiness
  • IPTG toxicity
  • Stability of output protein

The conclusions from the models should help people from the Wetlab to plan their experiments and take into account these limitations. This module is an integral part of the design, as large-scale commercialization of the drug of interest depends on finding the optimal conditions for protein production.

Model for drug enzyme kinetics

The protein of interest is an enzyme. It will bind to specific substrates and increase the rate of their conversion into products. Therefore, by monitoring either the substrate concentration or the product concentration, we can indirectly see the activity of the enzyme. This is quantified by measuring by enzyme activity, in the cellulase assay and PAH assay. Enzyme activity is the rate of substrate utilisation /product formation per unit time.

Our Goals

This model aims to better understand the enzymatic action of the drug by:

• Characterizing its enzymatic activity
• Subsequently,by modeling the relationship between the quantity of protein being produced and the enzyme activity using a simple Michaelis-Menten enzyme kinetics model will enable us to take into account factors that could impair enzymatic activity

This model will allow an understanding of the activity of the enzymes and allow the WETLAB to have an idea of the magnitude of the enzymatic activity. More importantly, after the results from enzyme assays are obtained, the model should provide a means of relating the activity output data to the concentration of the enzyme.