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Team:Calgary/9 June 2009
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| - | + | Preparation for Maxiprep | |
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| - | + | * Prepared overnight cultures for maxi-prep tomorrow. | |
| + | * Read through several modelling papers. | ||
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Isolated plasmid from LuxOD47E overnight cultures using Sigma miniprep kit, lot # 035K6068, eluting in ddH2O. Learned how to use Nanodrop Spectrophotometer to determine the concentration of the DNA. Prepared DNA for DNA sequecning. The optimal concentration of DNA for DNA sequencing is 100 ng/ 1 kb. Our gene is 1362 base pairs and the concentration is 292.3 ng/uL, so we sent down 1.8 uL of DNA with 7.2 uL ddH2O. | Isolated plasmid from LuxOD47E overnight cultures using Sigma miniprep kit, lot # 035K6068, eluting in ddH2O. Learned how to use Nanodrop Spectrophotometer to determine the concentration of the DNA. Prepared DNA for DNA sequecning. The optimal concentration of DNA for DNA sequencing is 100 ng/ 1 kb. Our gene is 1362 base pairs and the concentration is 292.3 ng/uL, so we sent down 1.8 uL of DNA with 7.2 uL ddH2O. | ||
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| - | + | Marketing and Outreach for June 9th 2009 | |
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| - | + | Today, I contacted some Oil and Gas companies via e-mail and I will make follow up phone calls soon. | |
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| + | I also signed up for second life ™, which would be our biggest part of our outreach aspect of our project. I wanted to familiarize myself with the online virtual world so I can get more ideas on how to hold the ethics conference in second life ™ | ||
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| - | + | Preparation of luxPQ in TOPO for DNA Sequencing | |
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| - | + | Two colonies of luxPQ in the TOPO vector were sent for sequencing, one from this year and one from 2008. If luxPQ is present in either colony, then this colony will be proceeded with in the project. As per the University of Calgary DNA Sequencing requirements, there was 100ng/uL of DNA and water up to 10uL. Sequencing primers included primers annealing to the 5’ end and 3’ end of the multiple cloning site. | |
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| - | + | A Lesson Learned in Second Life | |
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| - | + | Do not group deed objects! Today I accidently lost Katie's PCR script while we played around with object ownership. We now have a better understanding at least of how to share building projects and scripts without losing the ability to modify or copy them. Sometimes, however, Second Life can still make us lose these 'permissions', so keeping copies of everything is a must! | |
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| + | Fahd and Prima got their first taste of Second Life today, and were able to complete an in-world survey about prefered Hotel ammenities for Hong Kong University in order to get us the Linden dollars we needed to upload the textures used in the Virtual Lab. Using these textures, I made more lab equipment, doors, and cupboards to make the environment more realistic and interesting. | ||
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| + | Made multiple recolors of the logo so that it could be voted on by team members today (we had approximately 16 different shades of green!). | ||
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| - | + | Persistant Bugs | |
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| - | + | Retrospective Notebook: This entry was not written on this day, but derived later from working notes I made that day. | |
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| + | Briefly became very excited at the idea of turning PDB models of proteins into ingame objects, instead of using spheres or other simple shapes, but this fell by the side. | ||
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| + | Encountered some very persistant bugs in the scheme I was using for communication. I forget just what the problems were now, but just as soon as I had figured out why the RNAP wasn't getting the message I'd learn that the DNA parts were no longer learning each other's names properly... | ||
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| + | I also had a conversation with someone from Brazil, through one of second life's universal translators. Yes, you heard me right, it is only 2009 and we have (primitive) universal translators! Look up the Hank Ramos Universal Translator, for more. | ||
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| - | + | Introduction to Second Life | |
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| - | + | I signed up in Second Life today. My avatar's name is Synthiya Shieldmaiden. I explored our island, sat on pretty much anything that was shaking or moving. I also met my Second Life team members and spoke to them using the local chat box. I also enjoyed flying in Second Life. | |
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| + | After couple horus of exploring, I completed an online survey to raise Lynden dollars for the Second Life team. | ||
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Latest revision as of 16:42, 13 September 2009
UNIVERSITY OF CALGARY
