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Template:Team:KULeuven/10 September 2009/VanillinProduction
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JochemDeen (Talk | contribs) (New page: * Gel electrophoresis of TER restriction ** The vector for the TER is Psb1AK3 and has 3100bp, with insert is ±3200 bp ** Gel extraction {| border ="1" align="center" !| part || concentra...) |
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* Gel electrophoresis of TER restriction | * Gel electrophoresis of TER restriction | ||
| - | ** The vector for the TER is Psb1AK3 and has 3100bp, with insert | + | ** The vector for the TER is Psb1AK3 and has 3100bp, with an insert of ±3200 bp |
** Gel extraction | ** Gel extraction | ||
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| - | * Created a | + | * Created a motherplate from EF1 |
* Ligated EF restriction + TER restriction | * Ligated EF restriction + TER restriction | ||
* Transferred the SAMS 1 and SAMS 2 colonies to liquid medium | * Transferred the SAMS 1 and SAMS 2 colonies to liquid medium | ||
Latest revision as of 08:26, 14 September 2009
- Gel electrophoresis of TER restriction
- The vector for the TER is Psb1AK3 and has 3100bp, with an insert of ±3200 bp
- Gel extraction
| part | concentration | 260/280 | 260/230 |
|---|---|---|---|
| TER | 14,2 | 1,97 |
- Created a motherplate from EF1
- Ligated EF restriction + TER restriction
- Transferred the SAMS 1 and SAMS 2 colonies to liquid medium
