Template:Team:KULeuven/12 August 2009/VanillinReceptor

(Difference between revisions)

Latest revision as of 09:21, 14 September 2009

B,G and R were stored for further procedure. A failed again: there was no visible growth. This second time there was no problem with the competent cells because we used a commercial product instead of a self made. We assumed there was a problem with adding the polyA-tails to the PCR product because, when redoing the gelelectrophoresis, there were no errors with the PCR product.
We did a new PCR on the bacteria with the inserted plasmids for B,G and R to check if the gene was inserted correctly.
PCR was performed for W,X,Y and Z to isolate the genes.

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-* B,G and R were stored for further procedure. A failed again because there was no growth visible. This second time there was no problem with the competent cells because we used a commercial product and not self made. We assumed there was a problem with adding the polyA tales to the PCR product because we did a new gelelectroforesis and there were no errors with the PCR product.
+* B,G and R were stored for further procedure. A failed again: there was no visible growth. This second time there was no problem with the competent cells because we used a commercial product instead of a self made. We assumed there was a problem with adding the polyA-tails to the PCR product because, when redoing the gelelectrophoresis, there were no errors with the PCR product.
-*We did a new PCR on the bacteria with the inserted plasmids for B,G and R to check if the gene certainly was inserted correct.
+*We did a new PCR on the bacteria with the inserted plasmids for B,G and R to check if the gene was inserted correctly.
-*PCR was performed for W,X,Y and Z. The PCR product was purified and stored for further procedure
+*PCR was performed for W,X,Y and Z to isolate the genes.