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Template:Team:KULeuven/13 August 2009/VanillinReceptor
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| - | # B and R miniprepped. | + | # B and R miniprepped. Tomorrow proceed with mutagenesis of ''RpoA'' |
| - | # G redone (only | + | # G redone (only TOPO was visible, 200bp) PCR from 2,3 and 1' |
| - | # W, X, Y, Z | + | # W, X, Y, Z PCR purification to create a TOPO vector tomorrow |
Nanodrop: | Nanodrop: | ||
{| border ="1" align="center" | {| border ="1" align="center" | ||
Latest revision as of 09:22, 14 September 2009
- B and R miniprepped. Tomorrow proceed with mutagenesis of RpoA
- G redone (only TOPO was visible, 200bp) PCR from 2,3 and 1'
- W, X, Y, Z PCR purification to create a TOPO vector tomorrow
Nanodrop:
| Part | concentration (ng/μl) | 260/280 λ | 260/230 λ |
|---|---|---|---|
| W | 46,1 | 1,84 | |
| X | 112 | 1,82 | |
| Y | 207 | 1,85 | |
| Z | 155,3 | 1,86 |
