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Template:Team:KULeuven/2 September 2009/VanillinProduction
From 2009.igem.org
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* The four grown colonies are put on liquid culture | * The four grown colonies are put on liquid culture | ||
| - | * New (again...) restriction digest on | + | * New (again...) restriction digest on ''sam5, sam8, fcs'' and ''ech'', genes were miniprepped on Tuesday. |
| - | ** Overnight | + | ** Overnight at 16°C |
| - | * The plates with | + | * The plates with ''sam5, sam8, ech'' and ''fcs'' were re-ented. From ''sam5'' and ''fcs'' four colonies were picked and plated. |
* The PCR from yesterday did not deliver any results, probably because of an error when preparing the PCR mix. A new PCR was prepared and started today. | * The PCR from yesterday did not deliver any results, probably because of an error when preparing the PCR mix. A new PCR was prepared and started today. | ||
Latest revision as of 11:57, 14 September 2009
- The four grown colonies are put on liquid culture
- New (again...) restriction digest on sam5, sam8, fcs and ech, genes were miniprepped on Tuesday.
- Overnight at 16°C
- The plates with sam5, sam8, ech and fcs were re-ented. From sam5 and fcs four colonies were picked and plated.
- The PCR from yesterday did not deliver any results, probably because of an error when preparing the PCR mix. A new PCR was prepared and started today.
