Template:Team:KULeuven/7 September 2009/VanillinProduction

(Difference between revisions)

Latest revision as of 12:07, 14 September 2009

Miniprep of the four colonies from EF ligation
- Accidentally used double volumes of elution buffer on EF4

part	concentration	260/280	260/230
EF 1	115,5	1,98	2,31
EF 2	105,2	1,96	1,66
EF 3	95,1	2,05	0,98
EF 4	52,7	2,06	0,97

Gel electrophoresis from restriction on sam5 and sam8 looked good
Purified and ligated the restriction from sam5 and sam8 overnight
- Concentration of sam8 was barely enough for ligation...

part	concentration	260/280	260/230
sam5	10,1	2,11	0,04
sam8	3,1	3,96	0,02

@@ Line 1: / Line 1: @@
-* Miniprep from the four colonies of EF ligation
+* Miniprep of the four colonies from EF ligation
 ** Accidentally used double volumes of elution buffer on EF4
 {| border ="1" align="center"
@@ Line 8: / Line 8: @@
 | EF 2 || 105,2 || 1,96 || 1,66
 |- align="center"
-| EF 3 || 95,1 || 12,05 || 0,98
+| EF 3 || 95,1 || 2,05 || 0,98
 |- align="center"
 | EF 4 || 52,7 || 2,06 || 0,97
 |-
 |}
-* Gel electrophoresis of restriction from Sam5 and Sam8 looked good
+* Gel electrophoresis from restriction on ''sam5'' and ''sam8'' looked good
-* Purified and ligated the restriction from Sam5 and Sam8 overnight
+* Purified and ligated the restriction from ''sam5'' and ''sam8'' overnight
-** Concentration for sam8 was barely enough for ligation...
+** Concentration of ''sam8'' was barely enough for ligation...
 {| border ="1" align="center"
 !| part || concentration || 260/280 || 260/230
 |- align="center"
-| Sam5 || 10,1 || 2,11 || 0,04
+| ''sam5'' || 10,1 || 2,11 || 0,04
 |- align="center"
-| Sam8 || 3,1 || 3,96 || 0,02
+| ''sam8'' || 3,1 || 3,96 || 0,02
 |-
 |}