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Team:Imperial College London/Drylab/Protein Production
From 2009.igem.org
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Based on the [https://2009.igem.org/Team:Imperial_College_London/M1/Genetic Genetic circuit], a LacI-IPTG inducible promoter is responsible for kickstarting the production of the drug. | Based on the [https://2009.igem.org/Team:Imperial_College_London/M1/Genetic Genetic circuit], a LacI-IPTG inducible promoter is responsible for kickstarting the production of the drug. | ||
* In the absence of IPTG, LacI represses the production of the drug (Cellulase or PAH) | * In the absence of IPTG, LacI represses the production of the drug (Cellulase or PAH) | ||
Revision as of 13:53, 18 September 2009
- Overview
- The model
- Simulations
Protein Production
Based on the Genetic circuit, a LacI-IPTG inducible promoter is responsible for kickstarting the production of the drug.
- In the absence of IPTG, LacI represses the production of the drug (Cellulase or PAH)
- When IPTG is introduced, the LacI repressing pathway is “de-repressed”, and some output protein is produced.
Our goals
The modelling aims to provide an overview and better understanding of the M1 system’s function by:
- Characterizing the system.
- Modeling to account for several factors that may reduce/hinder the production of the protein drug such as:
- Lac promoter leakiness
- IPTG toxicity
- Stability of output protein
This module is an integral part of the design, as large-scale commercialization of the drug of interest depends on finding the optimal conditions for protein production. This model has also helped the Wetlab to plan some of the experiments.
