Team:UNIPV-Pavia/Methods Materials/Electrophoresis
From 2009.igem.org
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<table border='1'> | <table border='1'> | ||
<tr><td> | <tr><td> | ||
- | + | <font class='label'> | |
- | + | '''DNA samples'''<hr color='white' width='70%'> | |
- | + | '''Ethidium bromide'''<hr color='white' width='70%'> | |
- | + | '''Loading buffer 10x Blue Juice, Invitrogen'''<hr color='white' width='70%'> | |
+ | '''TBE (Tris/Borate/EDTA buffer) 5x (final volume 1 liter)'''<hr color='white' width='70%'> | ||
**'''54 gr Tris''' | **'''54 gr Tris''' | ||
**'''27.5 gr Borate''' | **'''27.5 gr Borate''' | ||
- | + | '''20 ml EDTA 0.5 M (pH 8)'''<hr color='white' width='70%'> | |
- | + | '''Marker (1 kb DNA Ladder, Promega)'''<hr color='white' width='70%'> | |
- | + | '''Face mask and gloves'''<hr color='white' width='70%'> | |
- | + | '''Electrophoresis apparatus'''<hr color='white' width='70%'> | |
- | + | '''Transilluminator'''</font> | |
</td></tr></table></div><br><br><br><br> | </td></tr></table></div><br><br><br><br> | ||
</td></tr></table> | </td></tr></table> |
Revision as of 16:31, 22 September 2009
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Electrophoresis
(estimated time: 2 hours)
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- NOTE: when not specified, the marker has the following pattern:
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