Team:UNIPV-Pavia/Methods Materials/Electrophoresis
From 2009.igem.org
(Difference between revisions)
(→Electrophoresis) |
(→Electrophoresis) |
||
Line 55: | Line 55: | ||
'''Loading buffer 10x Blue Juice, Invitrogen'''<hr color='white' width='70%'> | '''Loading buffer 10x Blue Juice, Invitrogen'''<hr color='white' width='70%'> | ||
'''TBE (Tris/Borate/EDTA buffer) 5x (final volume 1 liter)''' | '''TBE (Tris/Borate/EDTA buffer) 5x (final volume 1 liter)''' | ||
- | + | <br>*'''54 gr Tris''' | |
- | + | <br>*'''27.5 gr Borate'''<hr color='white' width='70%'> | |
'''20 ml EDTA 0.5 M (pH 8)'''<hr color='white' width='70%'> | '''20 ml EDTA 0.5 M (pH 8)'''<hr color='white' width='70%'> | ||
'''Marker (1 kb DNA Ladder, Promega)'''<hr color='white' width='70%'> | '''Marker (1 kb DNA Ladder, Promega)'''<hr color='white' width='70%'> |
Revision as of 16:33, 22 September 2009
![]() |
Electrophoresis
(estimated time: 2 hours)
|
|
- NOTE: when not specified, the marker has the following pattern:
![]() |