Team:Imperial College London/Wetlab/Protocols/Autoinduction

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Latest revision as of 15:01, 23 September 2009

Section	Assay	Overview and Aims
Promoter Characterisation	Secondary Carbon Source Diauxie Growth	To generate the diauxie growth curves, as well as normal growth curves for Top-10 for modelling To determine the best secondary carbon source for optimal growth
Auto-Induction	Glucose Time Delay	* Characterise the tunable time duration it takes before GFP expression (M2 activation)

@@ Line 1: / Line 1: @@
 {{Imperial/09/TemplateTop}}
 {{Imperial/09/Tabs/Main/Wetlab/Protocols}}
-==Glucose time delay==
+{| style="color:#CCC; background-color:#325d97;" cellpadding="6" cellspacing="0" border="3"
-===Aims===
+! Section
+! Assay
-* Characterise the tunable time duration it takes before GFP expression (M2 activation)
+! Overview and Aims
+|- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1"
-===Assay===
+| <b>Promoter Characterisation</b>
+| [[Team:Imperial_College_London/Wetlab/Protocols/SecondaryCarbon|Secondary Carbon Source Diauxie Growth]]
-*The cells will be grown until OD= 0.7.
+|
+*	To generate the diauxie growth curves, as well as normal growth curves for Top-10 for modelling
-*The RFP value will be monitered, although it is the GFP values that are more critical in this assay.
+*	To determine the best secondary carbon source for optimal growth
+|- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1"
-*OD and fluorescence data for GFP which can be converted in [http://partsregistry.org/cgi/measurement/new_batch.cgi Specific Promoter Units (SPUs)].
+| <b>Auto-Induction</b>
+| [[Team:Imperial_College_London/Wetlab/Protocols/Glucose delay|Glucose Time Delay]]
-*The secondary carbon source will be taken from the previous experiment (see Secondary carbon source selection for CRP promoter)
+| * Characterise the tunable time duration it takes before GFP expression (M2 activation)
+|}
-*The IPTG concentrations will be taken from the previous experiment (see Determining concentration of IPTG)
-[[Team:Imperial_College_London/Wetlab/Protocols/Glucose delay|See Glucose time delay for details]]
 {{Imperial/09/TemplateBottom}}