Team:Imperial College London/Wetlab/Protocols/M2

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|- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1"
|- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1"
| <b>EN1</b>: Colanic acid production amount
| <b>EN1</b>: Colanic acid production amount
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| [[Team:Imperial_College_London/Wetlab/Protocols/IPTGgrowth |IPTG Toxicity]]
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| [[Team:Imperial_College_London/Wetlab/Protocols/ColanicAcid| Colanic Acid]]
| To investigate the effect of our IPTG inducer on growth of our cultures.
| To investigate the effect of our IPTG inducer on growth of our cultures.
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* Measure growth rates at 28 degrees Celsius on minimal growth media so that subsequent testing timings etc. can be streamlined
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* Measurement of the % change in GFP fluorescence following acid incubation. <br>
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* Determine the effect of IPTG toxicity on growth w/o any protein production complications <br>
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|- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1"
|- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1"
| <b>EN2</b>: Colanic acid protection from pH
| <b>EN2</b>: Colanic acid protection from pH

Latest revision as of 15:39, 23 September 2009

Encapsulation (EN)

Section Assay Overview and Aims
EN1: Colanic acid production amount Colanic Acid To investigate the effect of our IPTG inducer on growth of our cultures.
  • Measurement of the % change in GFP fluorescence following acid incubation.
EN2: Colanic acid protection from pH Link Aims
EN3: Trehalose production Link Aims

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