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Team:Imperial College London/Wetlab/Protocols/Calibration
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| - | | * | + | | * To relate how the fluorescence observed can be correlated with number of GFP molecules <br> |
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| <b>CA3</b>:Internal GFP fluorescence | | <b>CA3</b>:Internal GFP fluorescence | ||
Revision as of 15:41, 23 September 2009
Calibration protocols (CA)
| Section | Assay | Overview and Aims |
|---|---|---|
| CA1:Calibration Curves | TOP10 Growth | * To produce a calibration curve to aid in the normalising of absorbance values. The relation of absorbance reading to number of cells varies with different cell strains. We are therefore doing one for Top-10. |
| CA2:External GFP fluorescence | Link | * To relate how the fluorescence observed can be correlated with number of GFP molecules |
| CA3:Internal GFP fluorescence | Link | * T |
