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Team:Imperial College London/Wetlab/Protocols/Calibration
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| <b>CA2</b>:External GFP fluorescence | | <b>CA2</b>:External GFP fluorescence | ||
| - | | [[ | + | | [[Imperial_College_London/Wetlab/Protocols/FluorEx| External fluorescence]] |
| - | | * To relate how the fluorescence observed can be correlated with number of GFP molecules <br> | + | | * To relate how the fluorescence observed can be correlated with number of purified GFP molecules <br> |
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| <b>CA3</b>:Internal GFP fluorescence | | <b>CA3</b>:Internal GFP fluorescence | ||
| - | | [[ | + | | [[Imperial_College_London/Wetlab/Protocols/FluorIn| Intracellular fluorescence]] |
| - | | * | + | | * To relate how various intracellular concentrations of GFP leads to different observed fluorescences. This allows us to relate measured fluorescence from cells to actual GFP number <br> |
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Latest revision as of 15:44, 23 September 2009
Calibration protocols (CA)
| Section | Assay | Overview and Aims |
|---|---|---|
| CA1:Calibration Curves | TOP10 Growth | * To produce a calibration curve to aid in the normalising of absorbance values. The relation of absorbance reading to number of cells varies with different cell strains. We are therefore doing one for Top-10. |
| CA2:External GFP fluorescence | External fluorescence | * To relate how the fluorescence observed can be correlated with number of purified GFP molecules |
| CA3:Internal GFP fluorescence | Intracellular fluorescence | * To relate how various intracellular concentrations of GFP leads to different observed fluorescences. This allows us to relate measured fluorescence from cells to actual GFP number |
