Team:Chiba/Notebook/Calendar/7 September 2009
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([[Team:Chiba/Notebook/Calendar/6_September_2009|6_September_2009]] <|>[[Team:Chiba/Notebook/Calendar/8_September_2009|8_September_2009]]) | ([[Team:Chiba/Notebook/Calendar/6_September_2009|6_September_2009]] <|>[[Team:Chiba/Notebook/Calendar/8_September_2009|8_September_2009]]) | ||
- | + | == Measurement of Receiver's Responce by AHL concentration (1)== | |
*Making AHL water solution | *Making AHL water solution | ||
We made 1 μM, 10 μM, 1 mM AHL water solution from 100 mM AHL water solution. | We made 1 μM, 10 μM, 1 mM AHL water solution from 100 mM AHL water solution. | ||
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- | * | + | *prior culture |
- | + | We did prior culture using glycerol stock(BW25113, ΔTdk,pCIA3-LuxR,pLux-GFP) . | |
+ | |||
+ | == Handwritten Lab Notebook == | ||
[[Image:Chiba_Labwork_07Sep09.jpg|200px]] | [[Image:Chiba_Labwork_07Sep09.jpg|200px]] |
Latest revision as of 17:50, 23 September 2009
(6_September_2009 <|>8_September_2009)
Measurement of Receiver's Responce by AHL concentration (1)
- Making AHL water solution
We made 1 μM, 10 μM, 1 mM AHL water solution from 100 mM AHL water solution.
- Making plates
Using each concentration of AHL water solutions which made ealier, we made two plates per the concentration.
- prior culture
We did prior culture using glycerol stock(BW25113, ΔTdk,pCIA3-LuxR,pLux-GFP) .