Team:Chiba/Notebook/Calendar/8 September 2009

From 2009.igem.org

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== Measurement of Receiver's Responce by AHL concentration (1)==
== Measurement of Receiver's Responce by AHL concentration (1)==
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*Inoculation
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=== 13:55 Inoculation===
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13:55
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We cultured a sample at 37 degrees Celsius.
We cultured a sample at 37 degrees Celsius.
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16:15
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===16:15 Observation of GFP fluorescence ===
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We transferred cultured sample into plate.
We transferred cultured sample into plate.
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*Observation of GFP fluorescence
 
We observed samples every 30 min.
We observed samples every 30 min.
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Here are pictures of samples.
Here are pictures of samples.
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==== 16:15 (0) ====
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*prior culture
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20:45
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We did prior culture using glycerol stock(XL10G, plac-LuxI, PSB1, Amp) .
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== Lab Notebook ==
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[[Image:Chiba_Labwork_08Sep09.jpg|200px]]
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== Pictures ==
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=== 16:15 (Start) ===
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<gallery>
<gallery>
Image:Chiba_0709_1615_0_nM.jpg|0 nM
Image:Chiba_0709_1615_0_nM.jpg|0 nM
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</gallery>
</gallery>
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=== 16:45 (1) ===
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==== 16:45 (1) ====
<gallery>
<gallery>
Image:Chiba_0709_1645_0_nM.jpg|0 nM
Image:Chiba_0709_1645_0_nM.jpg|0 nM
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</gallery>
</gallery>
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=== 17:15 (2)===
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==== 17:15 (2)====
<gallery>
<gallery>
Image:Chiba_0709_1715_0_nM.jpg|0 nM
Image:Chiba_0709_1715_0_nM.jpg|0 nM
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</gallery>
</gallery>
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=== 17:45 (3) ===
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==== 17:45 (3) ====
<gallery>
<gallery>
Image:Chiba_0709_1745_0_nM.jpg|0 nM
Image:Chiba_0709_1745_0_nM.jpg|0 nM
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</gallery>
</gallery>
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=== 18:15 (4) ===
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==== 18:15 (4) ====
<gallery>
<gallery>
Image:Chiba_0709_1815_0_nM.jpg|0 nM
Image:Chiba_0709_1815_0_nM.jpg|0 nM
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</gallery>
</gallery>
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=== 18:45 (5) ===
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==== 18:45 (5) ====
<gallery>
<gallery>
Image:Chiba_0709_1845_0_nM.jpg|0 nM
Image:Chiba_0709_1845_0_nM.jpg|0 nM
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</gallery>
</gallery>
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=== 19:15 (6) ===
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==== 19:15 (6) ====
<gallery>
<gallery>
Image:Chiba_0709_1915_0_nM.jpg|0 nM
Image:Chiba_0709_1915_0_nM.jpg|0 nM
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</gallery>
</gallery>
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=== 19:45 (7) ===
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==== 19:45 (7) ====
<gallery>
<gallery>
Image:Chiba_0709_1945_0_nM.jpg|0 nM
Image:Chiba_0709_1945_0_nM.jpg|0 nM
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Image:Chiba_0709_1945_1000_nM.jpg|1000 nM
Image:Chiba_0709_1945_1000_nM.jpg|1000 nM
</gallery>
</gallery>
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== Check of Sender's AHL generation (1) Day 1 ==
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==== 20:45 start prior culture ====
 +
We did prior culture using glycerol stock(XL10G, plac-LuxI, PSB1, Amp) .
 +
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== Handwritten Lab Notebook ==
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[[Image:Chiba_Labwork_08Sep09.jpg|200px]]

Latest revision as of 18:22, 23 September 2009

>Go to the Notebook page

(7_September_2009 <|>9_September_2009)


Contents

Measurement of Receiver's Responce by AHL concentration (1)

13:55 Inoculation

We cultured a sample at 37 degrees Celsius.


16:15 Observation of GFP fluorescence

We transferred cultured sample into plate. We observed samples every 30 min. Here are pictures of samples.

16:15 (0)

0 nM

1 nM

10 nM

100 nM

1000 nM

All

16:45 (1)

0 nM

1 nM

10 nM

100 nM

1000 nM

17:15 (2)

0 nM

1 nM

10 nM

100 nM

1000 nM

All

17:45 (3)

0 nM

1 nM

10 nM

100 nM

1000 nM

All

18:15 (4)

0 nM

1 nM

10 nM

100 nM

1000 nM

All

18:45 (5)

0 nM

1 nM

10 nM

100 nM

1000 nM

All

19:15 (6)

0 nM

1 nM

10 nM

100 nM

1000 nM

All

19:45 (7)

0 nM

1 nM

10 nM

100 nM

1000 nM

Check of Sender's AHL generation (1) Day 1

20:45 start prior culture

We did prior culture using glycerol stock(XL10G, plac-LuxI, PSB1, Amp) .

Handwritten Lab Notebook

Chiba Labwork 08Sep09.jpg

Retrieved from "http://2009.igem.org/Team:Chiba/Notebook/Calendar/8_September_2009"