Team:Chiba/Notebook/Calendar/8 September 2009
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== Measurement of Receiver's Responce by AHL concentration (1)== | == Measurement of Receiver's Responce by AHL concentration (1)== | ||
- | + | === 13:55 Inoculation=== | |
- | 13:55 | + | |
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We cultured a sample at 37 degrees Celsius. | We cultured a sample at 37 degrees Celsius. | ||
- | 16:15 | + | ===16:15 Observation of GFP fluorescence === |
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We transferred cultured sample into plate. | We transferred cultured sample into plate. | ||
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We observed samples every 30 min. | We observed samples every 30 min. | ||
Here are pictures of samples. | Here are pictures of samples. | ||
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Image:Chiba_0709_1945_1000_nM.jpg|1000 nM | Image:Chiba_0709_1945_1000_nM.jpg|1000 nM | ||
</gallery> | </gallery> | ||
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+ | == Check of Sender's AHL generation (1) Day 1 == | ||
+ | ==== 20:45 start prior culture ==== | ||
+ | We did prior culture using glycerol stock(XL10G, plac-LuxI, PSB1, Amp) . | ||
== Handwritten Lab Notebook == | == Handwritten Lab Notebook == | ||
[[Image:Chiba_Labwork_08Sep09.jpg|200px]] | [[Image:Chiba_Labwork_08Sep09.jpg|200px]] |
Latest revision as of 18:22, 23 September 2009
(7_September_2009 <|>9_September_2009)
Contents |
Measurement of Receiver's Responce by AHL concentration (1)
13:55 Inoculation
We cultured a sample at 37 degrees Celsius.
16:15 Observation of GFP fluorescence
We transferred cultured sample into plate. We observed samples every 30 min. Here are pictures of samples.
16:15 (0)
16:45 (1)
17:15 (2)
17:45 (3)
18:15 (4)
18:45 (5)
19:15 (6)
19:45 (7)
Check of Sender's AHL generation (1) Day 1
20:45 start prior culture
We did prior culture using glycerol stock(XL10G, plac-LuxI, PSB1, Amp) .