Team:Kyoto/CiC/Notebook

From 2009.igem.org

(Difference between revisions)
Line 25: Line 25:
<!-- ** Edit below ** -->
<!-- ** Edit below ** -->
 +
<html>
 +
 +
<style>
 +
#calendar{
 +
  width:100px;
 +
  border:2px #CCC solid;
 +
  background:#DDD;
 +
}
 +
#calendar ul{
 +
  padding:0;
 +
  margin:0;
 +
  width:100px;
 +
  list-style:none;
 +
}
 +
#calendar ul li{
 +
  padding:0;
 +
  margin:0;
 +
  width:100px;
 +
  height:30px;
 +
}
 +
#calendar ul li a{
 +
  display:block;
 +
  width:90px;
 +
  height:30px;
 +
  padding-left:10px;
 +
  text-decoration:none;
 +
  font-weight:bold;
 +
  line-height:30px;
 +
}
 +
 +
#calendar ul li a#calendar_week1{
 +
  background:#EEE;
 +
}
 +
 +
#calendar ul li a:link{
 +
}
 +
#calendar ul li a:hover{
 +
  background:#EEE;
 +
}
 +
</style>
<div id="rightcontents">
<div id="rightcontents">
-
==Goals==
+
<div id="calendar">
-
Below is the goals of this project.
+
<ul>
-
===Subgoal 1===
+
  <li><a id="calendar_week1" href="https://2009.igem.org/Team:Kyoto/Cells_in_Cells/Notebook/week1" title="1st week">1st week</a></li>
-
this is the subgoal.this is the subgoal.this is the subgoal.this is the subgoal.this is the subgoal.this is the subgoal.this is the subgoal.this is the subgoal.this is the subgoal.this is the subgoal.this is the subgoal.this is the subgoal.this is the subgoal.this is the subgoal.this is the subgoal.this is the subgoal.this is the subgoal.this is the subgoal.this is the subgoal.this is the subgoal.this is the subgoal.this is the subgoal.this is the subgoal.this is the subgoal.this is the subgoal.this is the subgoal.this is the subgoal.this is the subgoal.this is the subgoal.this is the subgoal.
+
  <li><a id="calendar_week2" href="https://2009.igem.org/Team:Kyoto/Cells_in_Cells/Notebook/week2" title="2nd week">2nd week</a></li>
-
===Subgoal 2===
+
  <li><a id="calendar_week3" href="https://2009.igem.org/Team:Kyoto/Cells_in_Cells/Notebook/week3" title="3rd week">3rd week</a></li>
-
this is the subgoal.this is the subgoal.this is the subgoal.this is the subgoal.this is the subgoal.this is the subgoal.this is the subgoal.this is the subgoal.this is the subgoal.this is the subgoal.this is the subgoal.this is the subgoal.this is the subgoal.this is the subgoal.this is the subgoal.this is the subgoal.this is the subgoal.this is the subgoal.this is the subgoal.this is the subgoal.this is the subgoal.this is the subgoal.this is the subgoal.this is the subgoal.this is the subgoal.this is the subgoal.this is the subgoal.this is the subgoal.this is the subgoal.this is the subgoal.
+
  <li><a id="calendar_week4" href="https://2009.igem.org/Team:Kyoto/Cells_in_Cells/Notebook/week4" title="4th week">4th week</a></li>
-
===Subgoal 3===
+
  <li><a id="calendar_week5" href="https://2009.igem.org/Team:Kyoto/Cells_in_Cells/Notebook/week5" title="5th week">5th week</a></li>
-
this is the subgoal.this is the subgoal.this is the subgoal.this is the subgoal.this is the subgoal.this is the subgoal.this is the subgoal.this is the subgoal.this is the subgoal.this is the subgoal.this is the subgoal.this is the subgoal.this is the subgoal.this is the subgoal.this is the subgoal.this is the subgoal.this is the subgoal.this is the subgoal.this is the subgoal.this is the subgoal.this is the subgoal.this is the subgoal.this is the subgoal.this is the subgoal.this is the subgoal.this is the subgoal.this is the subgoal.this is the subgoal.this is the subgoal.this is the subgoal.
+
  <li><a id="calendar_week6" href="https://2009.igem.org/Team:Kyoto/Cells_in_Cells/Notebook/week6" title="6th week">6th week</a></li>
 +
  <li><a id="calendar_week7" href="https://2009.igem.org/Team:Kyoto/Cells_in_Cells/Notebook/week7" title="7th week">7th week</a></li>
 +
  <li><a id="calendar_week8" href="https://2009.igem.org/Team:Kyoto/Cells_in_Cells/Notebook/week8" title="8th week">8th week</a></li>
 +
</ul>
 +
</div><!-- /#calendar -->
 +
</html>
 +
 
 +
===Monday===
 +
====To Do on 24 July====
 +
*Miniprep (3-O-2, 3-A-4)
 +
*Restriction Enzyme Digestion
 +
*Electrophoresis
 +
 
 +
====Results====
 +
=====Miniprep=====
 +
*3-O-2, 3-A-4 was minipreped.
 +
 
 +
=====Digestion=====
 +
*Digested with EcoR1 / EcoR1 and Pst1 and run on a gel.
 +
 
 +
{| border="1" style="text-align:center;width:50%"
 +
|+Sample list
 +
!NO.!!Sample Name!!Enzyme
 +
|-align="center"
 +
|1||3-O-2||Eco R1, PST1
 +
|-align="center"
 +
|2||3-A-4||Eco R1, PST1
 +
|-align="center"
 +
|3||3-O-2||Eco R1
 +
|-align="center"
 +
|4||3-A-4||Eco R1
 +
|}
 +
 
 +
=====Electrophoresis=====
 +
[[Image:Kyoto_e_090724_1.png|250px]]
 +
* Very clear bands were found. (See the gel image in right!)
 +
* The bands are listed below.
-
</div><!-- /#rightcontents -->
+
{| border="1" style="text-align:center;width:50%"
 +
|+Parts and Plasmid length
 +
!!!3-O-2!!3-A-4
 +
|-
 +
|Parts length / bp||2837||487
 +
|-
 +
|Plasmid length / bp||3266||3266
 +
|}
 +
<html></div><!-- /#rightcontents --></html>

Revision as of 09:31, 24 September 2009

  1. Home
  2. GSDD
  3. Notebook

Monday

To Do on 24 July

  • Miniprep (3-O-2, 3-A-4)
  • Restriction Enzyme Digestion
  • Electrophoresis

Results

Miniprep
  • 3-O-2, 3-A-4 was minipreped.
Digestion
  • Digested with EcoR1 / EcoR1 and Pst1 and run on a gel.
Sample list
NO.Sample NameEnzyme
13-O-2Eco R1, PST1
23-A-4Eco R1, PST1
33-O-2Eco R1
43-A-4Eco R1
Electrophoresis

Kyoto e 090724 1.png

  • Very clear bands were found. (See the gel image in right!)
  • The bands are listed below.
Parts and Plasmid length
3-O-23-A-4
Parts length / bp2837487
Plasmid length / bp32663266

Retrieved from "http://2009.igem.org/Team:Kyoto/CiC/Notebook"