Team:Chiba/Notebook/Calendar/26 September 2009

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6 : sfGFP
6 : sfGFP
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*Element of mixtures
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<table width="200" border="1" cellpadding="0"  cellspacing="0" bordercolor="#000000"><tr>
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<td width="100">Template</td>
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<td width="100">1 &mu;L</td>
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</tr>
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<tr>
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<td>Primer(F)</td>
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<td>2.5 &mu;L</td>
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</tr>
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<tr>
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<td>Primer(R)</td>
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<td>2.5 &mu;L</td>
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</tr>
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<tr>
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<td>Buffer(x 10)</td>
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<td>5 &mu;L</td>
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</tr>
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<tr>
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<td>dNTP</td>
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<td>4 &mu;L</td>
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</tr>
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<tr>
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<td>DNA pol.</td>
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<td>1 &mu;L</td>
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</tr>
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<tr>
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<td>dH<sub>2</sub>O</td>
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<td>34 &mu;L</td>
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</tr>
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<tr>
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<td>Total</td>
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<td>50 &mu;L</td>
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</tr>
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</table>

Revision as of 07:38, 26 September 2009

>Go to the Notebook page

(25_September_2009 <|>27_September_2009)


Examine limit of AHL generation(2)-2

Yesterday's operation is here.


  • Today's operation

11:35-

We transplanted E.coli to NC filter and put these on each plates.

And then we took pictures every 30 min.


Picture are here.


Making color

PCR

  • Template

1 : ECFP

2 : mCherry

3 : V-YFP

4 : mRFP without Nco1 site

5 : mOrange

6 : sfGFP


  • Element of mixtures
Template 1 μL
Primer(F) 2.5 μL
Primer(R) 2.5 μL
Buffer(x 10) 5 μL
dNTP 4 μL
DNA pol. 1 μL
dH2O 34 μL
Total 50 μL
Retrieved from "http://2009.igem.org/Team:Chiba/Notebook/Calendar/26_September_2009"