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Team:Chiba/Notebook/Calendar/26 September 2009
From 2009.igem.org
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=== PCR === | === PCR === | ||
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6 : sfGFP | 6 : sfGFP | ||
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| + | 14:45 | ||
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| + | We drew up mixtures for PCR. | ||
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| + | 15:20- | ||
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| + | PCR | ||
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| + | 15:30 | ||
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| + | We made gel for electrophoresis. | ||
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| + | agar 0.14 g | ||
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| + | TAE 20 mL | ||
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| + | 17:25- | ||
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| + | Gel electrophoresis(135 V, 27 min) | ||
Revision as of 08:46, 26 September 2009
(25_September_2009 <|>27_September_2009)
Examine limit of AHL generation(2)-2
Yesterday's operation is here.
- Today's operation
11:35-
We transplanted E.coli to NC filter and put these on each plates.
And then we took pictures every 30 min.
Picture are here.
Making colors
PCR
- Template
1 : ECFP
2 : mCherry
3 : V-YFP
4 : mRFP without Nco1 site
5 : mOrange
6 : sfGFP
14:45
We drew up mixtures for PCR.
- Element of mixtures
| Template | 1 μL |
| Primer(F) | 2.5 μL |
| Primer(R) | 2.5 μL |
| Buffer(x 10) | 5 μL |
| dNTP | 4 μL |
| DNA pol. | 1 μL |
| dH2O | 34 μL |
| Total | 50 μL |
15:20-
PCR
15:30
We made gel for electrophoresis.
agar 0.14 g
TAE 20 mL
17:25-
Gel electrophoresis(135 V, 27 min)
