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Team:Chiba/Notebook/Calendar/26 September 2009
From 2009.igem.org
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=== PCR === | === PCR === | ||
*Template | *Template | ||
| - | 1 : ECFP | + | 1 : ECFP([http://partsregistry.org/Part:BBa_I6057 BBa_I6057]) |
| - | 2 : mCherry | + | 2 : mCherry([http://partsregistry.org/Part:BBa_E2060 BBa_E2060]) |
| - | 3 : V-YFP | + | 3 : V-YFP([http://partsregistry.org/Part:BBa_K084003 BBa_K084003]) |
| - | 4 : | + | 4 : mOrange([http://partsregistry.org/Part:BBa_E2050 BBa_E2050]) |
| - | 5 : | + | 5 : LacZ α([http://partsregistry.org/Part:BBa_T9003 BBa_T9003]) |
| - | 6 : | + | 6 : mRFP without Nco1 site |
| Line 207: | Line 207: | ||
PCR | PCR | ||
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| - | |||
=== Gel electrophoresis === | === Gel electrophoresis === | ||
Revision as of 05:05, 27 September 2009
(25_September_2009 <|>27_September_2009)
Contents |
E.coli Painting(3)
 RFP, GFP. CFP |
Examine limit of AHL generation(2)-2
Yesterday's operation is here.
- Today's operation
11:35-
We transplanted E.coli to NC filter and put these on each plates.
And then we took pictures every 30 min.
Picture are here.
Pictures
11:35
 Negative Control |
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12:05
 Negative Control |
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12:35
 Negative Control |
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13:05
 Negative Control |
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13:35
 Negative Control |
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14:05
 Negative Control |
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14:35
 Negative Control |
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15:05
 Negative Control |
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Making colors
PCR
- Template
1 : ECFP([http://partsregistry.org/Part:BBa_I6057 BBa_I6057])
2 : mCherry([http://partsregistry.org/Part:BBa_E2060 BBa_E2060])
3 : V-YFP([http://partsregistry.org/Part:BBa_K084003 BBa_K084003])
4 : mOrange([http://partsregistry.org/Part:BBa_E2050 BBa_E2050])
5 : LacZ α([http://partsregistry.org/Part:BBa_T9003 BBa_T9003])
6 : mRFP without Nco1 site
14:45
We drew up mixtures for PCR.
- Element of mixtures
| Template | 1 μL |
| Primer(F) | 2.5 μL |
| Primer(R) | 2.5 μL |
| Buffer(x 10) | 5 μL |
| dNTP | 4 μL |
| DNA pol. | 1 μL |
| dH2O | 34 μL |
| Total | 50 μL |
15:20-
PCR
Gel electrophoresis
15:30
We made gel for electrophoresis.
agar 0.14 g
TAE 20 mL
17:25-
Gel electrophoresis(135 V, 27 min)
