Imperial College London/Notebook/23 September 2009

From 2009.igem.org

(Difference between revisions)
(→Testing team)
Line 15: Line 15:
[[Image:II09_HVD-GFP-run1.png|600px]]
[[Image:II09_HVD-GFP-run1.png|600px]]
 +
 +
===OD calibration (2nd run)===
 +
*50uL inoculation
 +
*Carried out on ice
 +
*Three dilutions: 10<sup>-6</sup>, 10<sup>-7</sup> and 10<sup>-8</sup>
 +
**10<sup>-7</sup> and 10<sup>-8</sup> produced too few colonies
 +
*Suggestions:
 +
**Inoculate 100uL
 +
**Lower dilution factors (10<sup>-4 to -6</sup>)
{{Imperial/09/TemplateBottom}}
{{Imperial/09/TemplateBottom}}

Revision as of 15:11, 1 October 2009
Nuri: - Plotted curves GFP/normalized OD - Observations: Control is dodgy... negative values came up, so need to re-do in wetlab! - Diauxic growth model: Writing up and reviewing model and simulations

Testing team

Harvard GFP (1st run)

  • Conclusions:
    • Raw fluorescence seems to increase with time at 37°C while, at 28°C, it seems to stay stable
  • Problems:
    • Did not run enough data points to see the fluorescence of the shifted sample reach a steady state
    • Cannot really conclude that the increase of fluorescence is due to an increase of expression as opposed to simple cell growth. This can be seen by the similarity of data on the normalised graph (bottom right, figure 1).

II09 HVD-GFP-run1.png

OD calibration (2nd run)

  • 50uL inoculation
  • Carried out on ice
  • Three dilutions: 10-6, 10-7 and 10-8
    • 10-7 and 10-8 produced too few colonies
  • Suggestions:
    • Inoculate 100uL
    • Lower dilution factors (10-4 to -6)

Mr. Gene   Geneart   Clontech   Giant Microbes

Retrieved from "http://2009.igem.org/Imperial_College_London/Notebook/23_September_2009"