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Imperial College London/Notebook/25 September 2009
From 2009.igem.org
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==Testing team== | ==Testing team== | ||
===OD calibration=== | ===OD calibration=== | ||
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*Suggestions: | *Suggestions: | ||
**Dilute using factors: 10<sup>-4</sup>, 10<sup>-5</sup> and 10<sup>-6</sup> with 100uL inoculation | **Dilute using factors: 10<sup>-4</sup>, 10<sup>-5</sup> and 10<sup>-6</sup> with 100uL inoculation | ||
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Latest revision as of 15:16, 1 October 2009
Testing team
OD calibration
- Carried out 10-4 and 10-6 dilutions
- Carried out on ice
- Used 3 OD values: 1, 0.7 and 0.4
- Conclusions:
- Too many colonies on 10-4, too few on 10-6, 10-5 seems to be the most appropriate
- Suggestions:
- Dilute using factors: 10-4, 10-5 and 10-6 with 100uL inoculation
