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Imperial College London/Notebook/2 October 2009
From 2009.igem.org
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====Conclusions==== | ====Conclusions==== | ||
| - | + | *After 3 hours on ice, the OD stays relatively constant | |
| - | + | **Shows that number of cells during OD measurement and during plating stay the same. Therefore, OD correlates with the number of cells that are plated. | |
====Suggestions/Improvements==== | ====Suggestions/Improvements==== | ||
Revision as of 19:10, 4 October 2009
Contents |
Testing
OD calibration 4th run 2nd Oct
Protocol considerations
- Cells were grown overnight in 10ml of M9 media
- The cells were allowed to grow until a high OD in the late morning, then placed in cold room
- OD of around 0.4 to 1.2 was made up, and subsequently diluted up to 10-4 to 10-6
- 100ul of cells were inoculated into each plate
- The OD of the overnight cells were measured again after 3 hours on ice to check if cell number changes during dilution process
Results
Waiting to be uploaded
Conclusions
- After 3 hours on ice, the OD stays relatively constant
- Shows that number of cells during OD measurement and during plating stay the same. Therefore, OD correlates with the number of cells that are plated.
Suggestions/Improvements
Waiting to be uploaded
