Team:Imperial College London/Wetlab/Protocols/M1

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(Protein Production (PP))
 
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=Encapsulation (EN)=
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=Protein Production (PP)=
{| style="color:#CCC; background-color:#325d97;" cellpadding="6" cellspacing="0" border="3"
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! Section
! Section
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! Overview and Aims
! Overview and Aims
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|- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1"
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| <b>EN1</b>: Colanic acid production amount
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| <b>PP1</b>: IPTG Toxicity
| [[Team:Imperial_College_London/Wetlab/Protocols/IPTGgrowth |IPTG Toxicity]]
| [[Team:Imperial_College_London/Wetlab/Protocols/IPTGgrowth |IPTG Toxicity]]
| To investigate the effect of our IPTG inducer on growth of our cultures.
| To investigate the effect of our IPTG inducer on growth of our cultures.
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* Determine the effect of IPTG toxicity on growth w/o any protein production complications <br>
* Determine the effect of IPTG toxicity on growth w/o any protein production complications <br>
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|- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1"
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| <b>EN2</b>: Colanic acid protection from pH
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| <b>PP2</b>: Lac characterisation
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| [[Team: | Link]]
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| [[Team:Imperial_College_London/Wetlab/Protocols/IPTG-RFP |Lac characterisation]]
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| Aims <br>
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|To investigate how RFP production induced by Lac promoter varies with IPTG <br>
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|- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1"
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| <b>EN3</b>: Trehalose production
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| <b>PP3</b>: Cellulase
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| [[Team: | Link]]
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| [[Team:Imperial_College_London/Wetlab/Protocols/Cellulase |Cellulase]]
| Aims <br>
| Aims <br>
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|- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1"
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| <b>PP4</b>: PAH
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| [[Team:Imperial_College_London/Wetlab/Protocols/PAH |PAH]]
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| Aims<br>
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|}
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Latest revision as of 21:49, 4 October 2009

Protein Production (PP)

Section Assay Overview and Aims
PP1: IPTG Toxicity IPTG Toxicity To investigate the effect of our IPTG inducer on growth of our cultures.
  • Measure growth rates at 28 degrees Celsius on minimal growth media so that subsequent testing timings etc. can be streamlined
  • Determine the effect of IPTG toxicity on growth w/o any protein production complications
PP2: Lac characterisation Lac characterisation To investigate how RFP production induced by Lac promoter varies with IPTG
PP3: Cellulase Cellulase Aims
PP4: PAH PAH Aims

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