Team:Imperial College London/Wetlab/Protocols/M1
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- | = | + | =Protein Production (PP)= |
{| style="color:#CCC; background-color:#325d97;" cellpadding="6" cellspacing="0" border="3" | {| style="color:#CCC; background-color:#325d97;" cellpadding="6" cellspacing="0" border="3" | ||
! Section | ! Section | ||
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! Overview and Aims | ! Overview and Aims | ||
|- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1" | |- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1" | ||
- | | <b> | + | | <b>PP1</b>: IPTG Toxicity |
| [[Team:Imperial_College_London/Wetlab/Protocols/IPTGgrowth |IPTG Toxicity]] | | [[Team:Imperial_College_London/Wetlab/Protocols/IPTGgrowth |IPTG Toxicity]] | ||
| To investigate the effect of our IPTG inducer on growth of our cultures. | | To investigate the effect of our IPTG inducer on growth of our cultures. | ||
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* Determine the effect of IPTG toxicity on growth w/o any protein production complications <br> | * Determine the effect of IPTG toxicity on growth w/o any protein production complications <br> | ||
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- | | <b> | + | | <b>PP2</b>: Lac characterisation |
- | | [[Team: | | + | | [[Team:Imperial_College_London/Wetlab/Protocols/IPTG-RFP |Lac characterisation]] |
- | | | + | |To investigate how RFP production induced by Lac promoter varies with IPTG <br> |
|- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1" | |- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1" | ||
- | | <b> | + | | <b>PP3</b>: Cellulase |
- | | [[Team: | | + | | [[Team:Imperial_College_London/Wetlab/Protocols/Cellulase |Cellulase]] |
| Aims <br> | | Aims <br> | ||
+ | |- style="color:#333; background-color:#CCCCFF;" cellpadding="6" cellspacing="0" border="1" | ||
+ | | <b>PP4</b>: PAH | ||
+ | | [[Team:Imperial_College_London/Wetlab/Protocols/PAH |PAH]] | ||
+ | | Aims<br> | ||
|} | |} | ||
+ | |||
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Latest revision as of 21:49, 4 October 2009
Protein Production (PP)
Section | Assay | Overview and Aims |
---|---|---|
PP1: IPTG Toxicity | IPTG Toxicity | To investigate the effect of our IPTG inducer on growth of our cultures.
|
PP2: Lac characterisation | Lac characterisation | To investigate how RFP production induced by Lac promoter varies with IPTG |
PP3: Cellulase | Cellulase | Aims |
PP4: PAH | PAH | Aims |