Team:Freiburg bioware/Notebook/April

From 2009.igem.org

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<li>two oligo's: one for binding one monomer, the second for binding the second monomer and to create dsDNA (one monomer has to be inactivated).</li>
<li>two oligo's: one for binding one monomer, the second for binding the second monomer and to create dsDNA (one monomer has to be inactivated).</li>
<li>one oligo: both binding sites of the monomers on one oligo (also one monomer has to be inactivated).</li>
<li>one oligo: both binding sites of the monomers on one oligo (also one monomer has to be inactivated).</li>
 +
</ul>
<br>
<br>
The 196 C-terminale aminoacids of FokI were used from other groups (see reference 1). Primersequences are also provided in the publication.<br>
The 196 C-terminale aminoacids of FokI were used from other groups (see reference 1). Primersequences are also provided in the publication.<br>

Revision as of 14:34, 8 October 2009

FREiGEM 2009

April


24th april 12:30 - 18:00 o'clock Manuel

Literaturrecherche zu FokI.
Entstandene Ideen:
  • three oligo's: two for binding the monomers and one to create a dsDNA where FokI can bind.
  • two oligo's: one for binding one monomer, the second for binding the second monomer and to create dsDNA (one monomer has to be inactivated).
  • one oligo: both binding sites of the monomers on one oligo (also one monomer has to be inactivated).

The 196 C-terminale aminoacids of FokI were used from other groups (see reference 1). Primersequences are also provided in the publication.
Reference 1: Freiburg09_FokI_Zn-Finger_1996.pdf

29th april 21o'clock Rüdiger

FokI bound to dsDNA







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