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Team:Imperial College London/Temporal Control/Autoinduction/Diauxic Growth
From 2009.igem.org
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When cAMP levels are low, the inactive CRP protein is unable to bind to the promoter. Therefore, transcription does not start. When cAMP levels are high, cAMP binds to inactive CRP protein and activates it. This active CRP protein will trigger the start of transcription. | When cAMP levels are low, the inactive CRP protein is unable to bind to the promoter. Therefore, transcription does not start. When cAMP levels are high, cAMP binds to inactive CRP protein and activates it. This active CRP protein will trigger the start of transcription. | ||
| - | + | <html><center><a href="https://2009.igem.org/Team:Imperial_College_London/Temporal_Control/Chemical_Induction"><img style="vertical-align:bottom;" width="20%" src="http://i691.photobucket.com/albums/vv271/dk806/II09_Drylabmaainimage1.png"></a><a href="https://2009.igem.org/Team:Imperial_College_London/Temporal_Control/Chemical_Induction"><img style="vertical-align:bottom;" width="20%" src="http://i691.photobucket.com/albums/vv271/dk806/II09_Drylabmainimage2.png"></a><a href="https://2009.igem.org/Team:Imperial_College_London/Temporal_Control/Thermoinduction "><img style="vertical-align:bottom;" width="20%" src="http://i691.photobucket.com/albums/vv271/dk806/II09_Drylabmainimage3.png"></a><a href="https://2009.igem.org/Team:Imperial_College_London/Drylab"><img style="vertical-align:bottom;" width="20%" src="http://i691.photobucket.com/albums/vv271/dk806/II09_Drylabmainimage4.png"></a><a href="https://2009.igem.org/Team:Imperial_College_London/Wetlab"><img style="vertical-align:bottom;" width="20%" src="http://i691.photobucket.com/albums/vv271/dk806/II09_Drylabmainimage5.png"></a></center></html> | |
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| + | <html><table border="0" style="background-color:transparent;" width="100%"> | ||
| + | <tr><td width="0%"> </td> | ||
| + | <td width="20%"><center><a href="https://2009.igem.org/Team:Imperial_College_London/Temporal_Control"><b>Temporal Control Overview</b></a></center></td> | ||
| + | <td width="20%"><center><a href="https://2009.igem.org/Team:Imperial_College_London/Temporal_Control/Chemical_Induction"><b>Chemical Induction</b></a></center></td> | ||
| + | <td width="20%"><center><a href="https://2009.igem.org/Team:Imperial_College_London/Temporal_Control/Thermoinduction"><b>Thermoinduction</b></a></center></td> | ||
| + | <td width="20%"><center><a href="https://2009.igem.org/Team:Imperial_College_London/Drylab"><b>Modelling</b></a></center></td> | ||
| + | <td width="20%"><center><a href="https://2009.igem.org/Team:Imperial_College_London/Wetlab"><b>Results</b></a></center></td> | ||
| + | <td width="1%"></td> | ||
| + | </tr></table></html> | ||
| + | <br> | ||
| + | ===References=== | ||
[1]J Monod-The growth of bacterial cultures- Annual Reviews in Microbiology, 1949 - Annual Reviews | [1]J Monod-The growth of bacterial cultures- Annual Reviews in Microbiology, 1949 - Annual Reviews | ||
{{Imperial/09/TemplateBottom}} | {{Imperial/09/TemplateBottom}} | ||
Revision as of 19:03, 8 October 2009
How it works- catabolite repression of promoter This is the phenomenon called catabolite repression, where a preferred carbon source represses other promoters by lowering intracellular cAMP levels. The key link here to the observed diauxic growth pattern is that the external glucose concentration is inversely correlated with the intracellular cAMP levels. For a CRP-linked promoter, transcription requires help from an active CRP protein (responsive to cAMP).
When cAMP levels are low, the inactive CRP protein is unable to bind to the promoter. Therefore, transcription does not start. When cAMP levels are high, cAMP binds to inactive CRP protein and activates it. This active CRP protein will trigger the start of transcription.
References
[1]J Monod-The growth of bacterial cultures- Annual Reviews in Microbiology, 1949 - Annual Reviews
