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Team:Heidelberg
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| - | ==''' | + | =='''iGEM Heidelberg Mission 2009: Spybricks'''== |
Establishing new standards for iGEM, the Heidelberg 2009 team will be concerned with developing ways for measuring promoters in mammalian cells, a default chassis and a first evaluation of the recently postulated BioBrick beta proposal 2 (Tom Knight). | Establishing new standards for iGEM, the Heidelberg 2009 team will be concerned with developing ways for measuring promoters in mammalian cells, a default chassis and a first evaluation of the recently postulated BioBrick beta proposal 2 (Tom Knight). | ||
Considering the importance of controlling gene expression, our team's work will focus on natural and synthetic mammalian promoters. Our vision is to provide the synthetic biology community with a methodical library of such promoters (with different output strength and sensitivity to different regulatory proteins) and a model which can provide guidance for the development of further synthetic promoters. Our efforts will therefore, from the very beginning, equally entail bioinformatics and wet lab work. | Considering the importance of controlling gene expression, our team's work will focus on natural and synthetic mammalian promoters. Our vision is to provide the synthetic biology community with a methodical library of such promoters (with different output strength and sensitivity to different regulatory proteins) and a model which can provide guidance for the development of further synthetic promoters. Our efforts will therefore, from the very beginning, equally entail bioinformatics and wet lab work. | ||
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Mammalian synthetic biology has huge potential, but it is in need of new standards and of systematic construction of comprehensive part libraries. Promoters are the fundamental elements of every synthetic biological system. We have developed and successfully applied two novel, in silico guided methods for the rational construction of synthetic promoters which respond only to predefined transcription factors. | Mammalian synthetic biology has huge potential, but it is in need of new standards and of systematic construction of comprehensive part libraries. Promoters are the fundamental elements of every synthetic biological system. We have developed and successfully applied two novel, in silico guided methods for the rational construction of synthetic promoters which respond only to predefined transcription factors. | ||
| - | [ | + | [ Please read more] |
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[[Image:HD09_iGEM_Modeling.png|center|150 px|border|Modeling]] | [[Image:HD09_iGEM_Modeling.png|center|150 px|border|Modeling]] | ||
'''The Heartbeat Database''' <br> | '''The Heartbeat Database''' <br> | ||
As one of the most important approaches to synthezise synthetic promotors we developed a database that predicts the position of conserved promotor binding sequences. We identified conserved sequences ourselves, analyzed the data and coded an easy to use interface that ise available for public use. | As one of the most important approaches to synthezise synthetic promotors we developed a database that predicts the position of conserved promotor binding sequences. We identified conserved sequences ourselves, analyzed the data and coded an easy to use interface that ise available for public use. | ||
| - | [ | + | [ Please read more] |
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'''Notebook''' <br> | '''Notebook''' <br> | ||
What's about documenation? You will find the entire progress of the project and all the important steps on our Notebook page. The Notebook is divided into the individual subgroups. | What's about documenation? You will find the entire progress of the project and all the important steps on our Notebook page. The Notebook is divided into the individual subgroups. | ||
| - | [ | + | [ Please read more] |
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'''Results'''<br> | '''Results'''<br> | ||
Mission Status: accomlished. We generated and characterized sets of constitutive and inducible promotors of different strength using our heartbeat-database prediction software and a new method developed in our lab. We combined a subset of these promotors with our targeted fluorescent protein tag and could thus show... Furthermore we characterized the eukaryotic standart | Mission Status: accomlished. We generated and characterized sets of constitutive and inducible promotors of different strength using our heartbeat-database prediction software and a new method developed in our lab. We combined a subset of these promotors with our targeted fluorescent protein tag and could thus show... Furthermore we characterized the eukaryotic standart | ||
| - | [ | + | [ Please read more] |
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'''Parts'''<br> | '''Parts'''<br> | ||
| - | Our team submits a library of well characterized and standardized promotors and sensors for eukaryotic cells. We will also contribute the first eucaryotic standart chassis for | + | Our team submits a library of well characterized and standardized promotors and sensors for eukaryotic cells. We will also contribute the first eucaryotic standart chassis for iGEM featuring standardized genome integration sites. |
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'''Gallery'''<br> | '''Gallery'''<br> | ||
Spy on our cells or join the Heidelberg Team in the lab with our gallery tour. | Spy on our cells or join the Heidelberg Team in the lab with our gallery tour. | ||
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'''Sponsors''' | '''Sponsors''' | ||
This year we have a lot of distinguished sponsors, which support us and this project. | This year we have a lot of distinguished sponsors, which support us and this project. | ||
| - | [ | + | [ Please read more] |
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'''Misc'''<br> | '''Misc'''<br> | ||
Valencia Gold Medal... | Valencia Gold Medal... | ||
| - | [ | + | [ Please read more] |
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Revision as of 10:53, 9 October 2009
iGEM Heidelberg Mission 2009: SpybricksEstablishing new standards for iGEM, the Heidelberg 2009 team will be concerned with developing ways for measuring promoters in mammalian cells, a default chassis and a first evaluation of the recently postulated BioBrick beta proposal 2 (Tom Knight). Considering the importance of controlling gene expression, our team's work will focus on natural and synthetic mammalian promoters. Our vision is to provide the synthetic biology community with a methodical library of such promoters (with different output strength and sensitivity to different regulatory proteins) and a model which can provide guidance for the development of further synthetic promoters. Our efforts will therefore, from the very beginning, equally entail bioinformatics and wet lab work.
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Our Team
This year 13 students are part of the Heidelberg iGEM Team.
News28 Aug. 2009, Generous Donation by Klaus Tschira Foundation 
The Klaus Tschira Foundation GmbH is a non-profit organization and supports research in informatics, the natural sciences, and mathematics; at the same time it promotes a public understanding in these sciences. With this combination the Klaus Tschira foundation is our ideal partner in this competition.
25 Aug. 2009, LANGE + PFLANZ becomes Gold Sponsor
http://igem.bioquant.uni-heidelberg.de/mediawiki/index.php/News See more |
