Team:British Columbia/Project

From 2009.igem.org

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<!--- The Mission, Experiments --->
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{{Team:British_Columbia/Templates/Header}}
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!align="center"|[[Team:Example|Home]]
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!align="center"|[[Team:Example/Team|The Team]]
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!align="center"|[[Team:Example/Project|The Project]]
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(''Or you can choose different headings.  But you must have a team page, a project page, and a notebook page.'')
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== '''Overall project''' ==
== '''Overall project''' ==
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1. Sensitivity of a biosensor
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Your abstract
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2. Logic gates
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== Project Details==
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=== Part 2 ===
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== Biosensor ==
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Using a random mutagenesis method directed at the promoter binding sites, we intend to construct a library of varying strength pBAD promoters coupled to a GFP reporter gene. Sequencing of select mutated promoters will result in a collection of pBAD variants that cover a wide range promoter sensitivity.
=== The Experiments ===
=== The Experiments ===
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1. Random mutagenesis of pBAD promoter sequence.
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2. Quantification of mutant promoter-driven GFP fluorescence.
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3. Sequencing of select mutants.
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4. BioBrick design and submission.
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=== Results ===
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== Logic Gates ==
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=== Part 3 ===
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== Results ==
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Revision as of 08:19, 30 March 2009

You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing.
Example logo.png

Tell us more about your project. Give us background. Use this is the abstract of your project. Be descriptive but concise (1-2 paragraphs)

Team.png
Team Example 2


Home The Team The Project Parts Submitted to the Registry Modeling Sponsor Us! Calendar Biosensor Sensitivity Notebook Biosensor Logic Gate Notebook

Contents

Overall project

1. Sensitivity of a biosensor

2. Logic gates

Biosensor

Using a random mutagenesis method directed at the promoter binding sites, we intend to construct a library of varying strength pBAD promoters coupled to a GFP reporter gene. Sequencing of select mutated promoters will result in a collection of pBAD variants that cover a wide range promoter sensitivity.

The Experiments

1. Random mutagenesis of pBAD promoter sequence. 2. Quantification of mutant promoter-driven GFP fluorescence. 3. Sequencing of select mutants. 4. BioBrick design and submission.

Results

Logic Gates

Retrieved from "http://2009.igem.org/Team:British_Columbia/Project"