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Team:Imperial College London/Drylab/Enzyme/Simulations
From 2009.igem.org
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| + | The above simulation shows that the Enzyme-Substrate complex is at a steady state. | ||
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| + | There is a decrease in substrate concentration, accompanied by a rise in product concentration. As all the substrate is used up, there will be no more products formed. There is, however, a time delay before significant product formation starts. This time delay occurs as the enzymatic conversion of substrate to product takes some time. | ||
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| + | After this time delay, the concentration of ES complex will gradually drop to 0, while the concentration of enzyme will correspondingly return to initial values. | ||
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Revision as of 21:38, 9 October 2009
Simulation 1: The Generic Graph
This simulation shows the standard enzyme kinetics graph. Concentrations of product, enzyme, substrate, and enzyme-substrate complex over time are shown. Comparisons between the standard enzyme kinetic graph with and without Michaelis-Menten kinetics are made.
In this simulation, all parameters are arbitrary. K1 = 100000, K2 = 1000 and K3 = 0.1. This makes KM = 0.01. Furthermore, [E0] = 0.01 and [S0] = 0.1. The values of K1, K2 and K3 are proportional to their kinetic values [1], while the values of E0 and S0 are chosen to ensure a clear graph.
The above simulation shows that the Enzyme-Substrate complex is at a steady state.
There is a decrease in substrate concentration, accompanied by a rise in product concentration. As all the substrate is used up, there will be no more products formed. There is, however, a time delay before significant product formation starts. This time delay occurs as the enzymatic conversion of substrate to product takes some time.
After this time delay, the concentration of ES complex will gradually drop to 0, while the concentration of enzyme will correspondingly return to initial values.
