"

 

From 2009.igem.org

Revision as of 23:22, 15 August 2009 (view source) Tadasi (Talk | contribs) (New page: ==miniprep==) ← Older edit		Latest revision as of 07:53, 12 October 2009 (view source) Unocchi (Talk | contribs)
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-	==miniprep==	+	===miniprep===
		+	Conducted a "miniprep" to harvest and check concentration of plasmids from 14 sample.Result of Nano drop concentration check :(sample No.) (concentration) [260/280] [260/230]
		+
		+	[http://partsregistry.org/wiki/index.php?title=Part:BBa_R0071 R0071] (1-12C)  29.5ng/μL　 1.56  0.91
		+	[http://partsregistry.org/wiki/index.php?title=Part:BBa_R0040 R0040] (1-6I)    7.5        1.88  2.79
		+	[http://partsregistry.org/wiki/index.php?title=Part:BBa_R0079 R0079] (1-12A)  11.5      1.92  2.65
		+	[http://partsregistry.org/wiki/index.php?title=Part:BBa_I14017 I14017] (1-18L)  28.6      1.81  2.46
		+	[http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 B0034] (1-2M)    10.1      1.96  2.60
		+	[http://partsregistry.org/wiki/index.php?title=Part:BBa_R0078 R0078] (1-16G)  14.0      1.99  2.47
		+	[http://partsregistry.org/wiki/index.php?title=Part:BBa_C0171 C0171] (2-8I)    15.4      1.86  3.29
		+	[http://partsregistry.org/wiki/index.php?title=Part:BBa_F2621 F2621] (2-21F)  17.9      1.99  2.60
		+	[http://partsregistry.org/wiki/index.php?title=Part:BBa_I14015 I14015] (2-11N)  11.2      1.88  2.32
		+	[http://partsregistry.org/wiki/index.php?title=Part:BBa_F1610 F1610] (2-24G)  19.5      1.97  2.34
		+	[http://partsregistry.org/wiki/index.php?title=Part:BBa_C0161 C0161] (2-8O)    11.0      1.60  1.74
		+	[http://partsregistry.org/wiki/index.php?title=Part:BBa_F2620 F2620] (2-6E)    5.5        1.46  2.03
		+	K204001 - 3    16.1      1.78  2.40
		+	K204001 - 8    14.4      1.77  2.38
		+
		+	===transformation===
		+	to get new plasmid
		+
		+	sample No.
		+	③　④　⑤　⑥
		+
		+	[https://2009.igem.org/Team:Osaka/NOTES back to NOTES]

---

Latest revision as of 07:53, 12 October 2009

miniprep

Conducted a "miniprep" to harvest and check concentration of plasmids from 14 sample.Result of Nano drop concentration check :(sample No.) (concentration) [260/280] [260/230]

  [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0071 R0071] (1-12C)   29.5ng/μL　 1.56  0.91
  [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0040 R0040] (1-6I)     7.5        1.88  2.79
  [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0079 R0079] (1-12A)   11.5       1.92  2.65
  [http://partsregistry.org/wiki/index.php?title=Part:BBa_I14017 I14017] (1-18L)   28.6       1.81  2.46
  [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 B0034] (1-2M)    10.1       1.96  2.60
  [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0078 R0078] (1-16G)   14.0       1.99  2.47
  [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0171 C0171] (2-8I)     15.4       1.86  3.29
  [http://partsregistry.org/wiki/index.php?title=Part:BBa_F2621 F2621] (2-21F)   17.9       1.99  2.60
  [http://partsregistry.org/wiki/index.php?title=Part:BBa_I14015 I14015] (2-11N)   11.2       1.88  2.32
  [http://partsregistry.org/wiki/index.php?title=Part:BBa_F1610 F1610] (2-24G)   19.5       1.97  2.34
  [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0161 C0161] (2-8O)    11.0       1.60  1.74
  [http://partsregistry.org/wiki/index.php?title=Part:BBa_F2620 F2620] (2-6E)     5.5        1.46  2.03
  K204001 - 3    16.1       1.78  2.40
  K204001 - 8    14.4       1.77  2.38

transformation

to get new plasmid

   sample No.
    ③　④　⑤　⑥

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