"
August/14 August 2009
From 2009.igem.org
(Difference between revisions)
(→miniprep) |
|||
| (3 intermediate revisions not shown) | |||
| Line 2: | Line 2: | ||
Conducted a "miniprep" to harvest and check concentration of plasmids from 14 sample.Result of Nano drop concentration check :(sample No.) (concentration) [260/280] [260/230] | Conducted a "miniprep" to harvest and check concentration of plasmids from 14 sample.Result of Nano drop concentration check :(sample No.) (concentration) [260/280] [260/230] | ||
| - | 1-12C 29.5 | + | [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0071 R0071] (1-12C) 29.5ng/μL 1.56 0.91 |
| + | [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0040 R0040] (1-6I) 7.5 1.88 2.79 | ||
| + | [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0079 R0079] (1-12A) 11.5 1.92 2.65 | ||
| + | [http://partsregistry.org/wiki/index.php?title=Part:BBa_I14017 I14017] (1-18L) 28.6 1.81 2.46 | ||
| + | [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 B0034] (1-2M) 10.1 1.96 2.60 | ||
| + | [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0078 R0078] (1-16G) 14.0 1.99 2.47 | ||
| + | [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0171 C0171] (2-8I) 15.4 1.86 3.29 | ||
| + | [http://partsregistry.org/wiki/index.php?title=Part:BBa_F2621 F2621] (2-21F) 17.9 1.99 2.60 | ||
| + | [http://partsregistry.org/wiki/index.php?title=Part:BBa_I14015 I14015] (2-11N) 11.2 1.88 2.32 | ||
| + | [http://partsregistry.org/wiki/index.php?title=Part:BBa_F1610 F1610] (2-24G) 19.5 1.97 2.34 | ||
| + | [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0161 C0161] (2-8O) 11.0 1.60 1.74 | ||
| + | [http://partsregistry.org/wiki/index.php?title=Part:BBa_F2620 F2620] (2-6E) 5.5 1.46 2.03 | ||
| + | K204001 - 3 16.1 1.78 2.40 | ||
| + | K204001 - 8 14.4 1.77 2.38 | ||
| + | |||
| + | ===transformation=== | ||
| + | to get new plasmid | ||
| + | |||
| + | sample No. | ||
| + | ③ ④ ⑤ ⑥ | ||
| + | |||
| + | [https://2009.igem.org/Team:Osaka/NOTES back to NOTES] | ||
Latest revision as of 07:53, 12 October 2009
miniprep
Conducted a "miniprep" to harvest and check concentration of plasmids from 14 sample.Result of Nano drop concentration check :(sample No.) (concentration) [260/280] [260/230]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_R0071 R0071] (1-12C) 29.5ng/μL 1.56 0.91 [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0040 R0040] (1-6I) 7.5 1.88 2.79 [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0079 R0079] (1-12A) 11.5 1.92 2.65 [http://partsregistry.org/wiki/index.php?title=Part:BBa_I14017 I14017] (1-18L) 28.6 1.81 2.46 [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 B0034] (1-2M) 10.1 1.96 2.60 [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0078 R0078] (1-16G) 14.0 1.99 2.47 [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0171 C0171] (2-8I) 15.4 1.86 3.29 [http://partsregistry.org/wiki/index.php?title=Part:BBa_F2621 F2621] (2-21F) 17.9 1.99 2.60 [http://partsregistry.org/wiki/index.php?title=Part:BBa_I14015 I14015] (2-11N) 11.2 1.88 2.32 [http://partsregistry.org/wiki/index.php?title=Part:BBa_F1610 F1610] (2-24G) 19.5 1.97 2.34 [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0161 C0161] (2-8O) 11.0 1.60 1.74 [http://partsregistry.org/wiki/index.php?title=Part:BBa_F2620 F2620] (2-6E) 5.5 1.46 2.03 K204001 - 3 16.1 1.78 2.40 K204001 - 8 14.4 1.77 2.38
transformation
to get new plasmid
sample No.
③ ④ ⑤ ⑥
