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Imperial College London/Notebook/6 October 2009
From 2009.igem.org
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| - | [[Image:II09_Harvard GFP 0610fluor.jpg]] | + | [[Image:II09_Harvard GFP 0610fluor.jpg|400px]] |
| - | [[Image:II09_Harvard GFP 0610Abs.jpg]] | + | [[Image:II09_Harvard GFP 0610Abs.jpg|400px]] |
| - | [[Image:II09_Harvard GFP 0610fluor-norm.jpg]] | + | [[Image:II09_Harvard GFP 0610fluor-norm.jpg|400px]] |
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Revision as of 12:21, 12 October 2009
Contents |
Dineka
- Midi on RcsB pst1
- Midi on PAH
- Mini's (x12) on OP 1-6 and OP+His 1-6
- Restriction Digest on Dam New 1-8 as sequencing failed 1st time round - wrong DNA sent?!
- Midi culture set off overnight for CRP-RBS+AK3 colony 1 from replica plate 17/9/09.
- Housekeeping - tips (all sizes), 1.5ml epis, 2ml epis, and pcr tubes sent to autoclave.
Testing
Harvard-GFP 6th Oct
Protocol considerations
- Cells were grown overnight in 5ml of M9 media
- Cells were then diluted and grown at 28 and 42 degrees
- The cells were allowed to acclimatise to their respective temperatures for 3 hours before the temperature shift took place
Results
Conclusions
- After 3 hours on ice, the OD stays relatively constant
- Shows that number of cells during OD measurement and during plating stay the same. Therefore, OD correlates with the number of cells that are plated.
Suggestions/Improvements
Waiting to be uploaded
Harvard-GFP
