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Imperial College London/Notebook/6 October 2009
From 2009.igem.org
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| - | + | ==Dineka== | |
* Midi on RcsB pst1 | * Midi on RcsB pst1 | ||
* Midi on PAH | * Midi on PAH | ||
* Mini's (x12) on OP 1-6 and OP+His 1-6 | * Mini's (x12) on OP 1-6 and OP+His 1-6 | ||
| - | * Restriction Digest on Dam New 1-8 as sequencing failed 1st time round - | + | * Restriction Digest on Dam New 1-8 as sequencing failed 1st time round - wrong DNA sent?! |
| - | * Midi culture set off overnight for CRP-RBS+AK3 colony 1 from replica plate 17/9/09 | + | * Midi culture set off overnight for CRP-RBS+AK3 colony 1 from replica plate 17/9/09. |
* Housekeeping - tips (all sizes), 1.5ml epis, 2ml epis, and pcr tubes sent to autoclave. | * Housekeeping - tips (all sizes), 1.5ml epis, 2ml epis, and pcr tubes sent to autoclave. | ||
| + | |||
| + | ==Testing== | ||
| + | ===Harvard-GFP 6th Oct=== | ||
| + | |||
| + | ====Protocol considerations==== | ||
| + | |||
| + | *Cells were grown overnight in 5ml of M9 media | ||
| + | *Cells were then diluted and grown at 28 and 42 degrees | ||
| + | *The cells were allowed to acclimatise to their respective temperatures for 3 hours before the temperature shift took place | ||
| + | |||
| + | |||
| + | ====Results==== | ||
| + | |||
| + | [[Image:II09_Harvard GFP 0610fluor.jpg|600px]] | ||
| + | [[Image:II09_Harvard GFP 0610Abs.jpg|600px]] | ||
| + | [[Image:II09_Harvard GFP 0610fluor-norm.jpg|600px]] | ||
| + | |||
| + | <br> | ||
| + | [[Media:Hvd-GFP 06-10.xls| Download raw data]] | ||
| + | |||
| + | ====Conclusions==== | ||
| + | |||
| + | Waiting for data analysis | ||
| + | |||
| + | ====Suggestions/Improvements==== | ||
| + | |||
| + | Waiting to be uploaded | ||
| + | |||
| + | |||
{{Imperial/09/TemplateBottom}} | {{Imperial/09/TemplateBottom}} | ||
Latest revision as of 12:23, 12 October 2009
Contents |
Dineka
- Midi on RcsB pst1
- Midi on PAH
- Mini's (x12) on OP 1-6 and OP+His 1-6
- Restriction Digest on Dam New 1-8 as sequencing failed 1st time round - wrong DNA sent?!
- Midi culture set off overnight for CRP-RBS+AK3 colony 1 from replica plate 17/9/09.
- Housekeeping - tips (all sizes), 1.5ml epis, 2ml epis, and pcr tubes sent to autoclave.
Testing
Harvard-GFP 6th Oct
Protocol considerations
- Cells were grown overnight in 5ml of M9 media
- Cells were then diluted and grown at 28 and 42 degrees
- The cells were allowed to acclimatise to their respective temperatures for 3 hours before the temperature shift took place
Results
Conclusions
Waiting for data analysis
Suggestions/Improvements
Waiting to be uploaded
