METU-gene/Human Practice

From 2009.igem.org

(Difference between revisions)

Revision as of 09:38, 13 October 2009

we are planning to prepare layers of wound- band. Wound- band consists of four layers. -At the top,there will be a polyuretan layer and this layer protects our bacteria and other layers of wound-band. The other property of this layer is being permable to gas from outside to inside,or vice versa. -In second layer, there will be LB Agar and lyophilized bacteria. The importance of this layer is to adjust concetration of gel as much as required.We are planning to adjust the size of its pore to transform just small proteins. -At the third layer, there will be a polyuretan,which is thinner than at the top.The pore size of this layer is adjusted to make easier the transformation of requiered proteins, not bacteria.Moreover this layer contains gelatin.I think we should express the properties of this gelatine. Firstly, this gelatine is very similar to collagen. But actually, it has more advantage than collogen. Firstly, gelatine is obtained much easier.Also, it is more economic.Moreover, while the over amount of collagen gives disadvantage for organism, the over amount of gelatine is not so important. Furthermore, gelatine has a high capacity to absorb substance.This property is important in EGF and KGF absorption. Another property is since this layer contact with wound, gelatine has biodegradable character.In other word, when it contacts with skin, it will be degraded.Moreover, may be the most important property of this layer is having a function as protein inhibitor

If we add directly EGF and KGF onto the open-wounded skin, since skin has proteaz enzyme, these proteins could be digested. As a result, we couldn’t provide any help to healing process.Thanks to gelatine layer, EGF and KGF are protected. In addition, we are planning to add microcapsules which contain granulysin. With the help of granulysin, the wound region will be sterilized. Basically, the main purpose of our project is to construct a mechanism in order to protect and to provide fast healing for wounds by using wound-band.This aim is started with EGF and KGF, but will be continued with the addition of other important proteins..

@@ Line 1: / Line 1: @@
+we are planning to prepare  layers of wound- band. Wound- band consists of four layers.
+-At the top,there will be a polyuretan layer and this layer protects our bacteria and other layers of wound-band. The other property of this layer is being permable to gas from outside to inside,or vice versa.
+-In second layer, there will be LB Agar and lyophilized bacteria. The importance of this layer is to adjust concetration of gel as much as required.We are planning to adjust the size of its pore to transform just small proteins.
+-At the third layer, there will be a polyuretan,which is thinner than at the top.The pore size of this layer is adjusted to make easier the transformation of requiered proteins, not bacteria.Moreover this layer contains  gelatin.I think we should express the properties of this gelatine. Firstly, this gelatine is very similar to collagen. But actually, it has more advantage than collogen. Firstly, gelatine is  obtained much easier.Also, it is more economic.Moreover, while the over amount of collagen gives disadvantage for organism, the over amount of gelatine is not so important. Furthermore, gelatine has a high capacity to absorb substance.This property is important in EGF and KGF absorption. Another property is since this layer contact with wound, gelatine has biodegradable character.In other word, when it contacts with skin, it will be degraded.Moreover, may be the most important property of this layer is having a function  as protein inhibitor
 <div align="center" style="padding-left: 66px; padding-top: 8px; ">https://static.igem.org/mediawiki/2009/3/32/Wounddressinglayers.jpg
 </div>
+If we add directly EGF and KGF onto the open-wounded skin, since skin has proteaz enzyme, these proteins could be digested. As a result, we couldn’t provide any help to healing process.Thanks to gelatine layer, EGF and KGF are protected. In addition, we are planning to  add microcapsules which contain granulysin. With the help of granulysin, the wound region will be sterilized.
+Basically, the main purpose of our project is to construct a mechanism in order to protect and to provide fast healing for wounds by using wound-band.This aim is started with EGF and KGF, but will be continued with the addition of other important proteins..