Team:IPN-UNAM-Mexico/Notebook/August 7 2009

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                                  <td><p align="right">&nbsp;</p>
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                                    <p align="right"><img src="https://static.igem.org/mediawiki/2009/a/a6/LabIconMX.png" alt="LabNotebook" width="30" height="35"> <span><a href="https://2009.igem.org/Team:IPN-UNAM-Mexico/Notebook">Back to Notebook</a></span></p>
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                                    <p><strong>August 7th, 2009</strong></p>
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                                    <p align="left" class="style8"><br>
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                                      Digestion Digestion using EcoRI : </p>
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                                    <ul>
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                                      <li>plasmidicDNA..........3&mu;l</li>
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                                      <li>Buffer 2....................1&mu;l&nbsp;&nbsp;&nbsp;&nbsp;  x30</li>
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                                      <li>BSA..........................0.5&mu;l&nbsp;  x30</li>
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                                      <li>EcoRI enzyme......... 0.5&mu;l&nbsp;&nbsp; x30</li>
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                                      <li>H2O..........................5&mu;l</li>
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                                    </ul>
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                                    <blockquote>&nbsp;</blockquote>
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                                    <p align="left" class="style8">An electrophoresis gel was ran with the following quantities:</p>
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<ul>
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                          <li>3&mu;l marker 1kb</li>
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                                      <li> Buffer 2 ................ 1&mu;l.</li>
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                          <li>3&mu;l DNA+ 3&mu;l of colorant</li>
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                          </ul>
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                                    <p>All samples were digested with EcoRI in order to linearized them.</p>
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                                    <p>Line 1 1kb | 3.1 ....* 3.4|9.1....* 9.9|9.8..  1|1kb|*18.2....18.4|19.1*....19.4|1kb</p>
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                                    <p>line&nbsp; 2 1kb|22.1.......*22.4|24.2&rdquo;&nbsp;&nbsp; 16.2&rdquo; 14.1 9.4|1kb</p>
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                                    <p>the ones marked with * are plasmids extracted today.</p>
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                                    <p align="left" >&nbsp;</p>
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                                    <p align="left" >&nbsp;</p>
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                                        <td width="50%"><a href="https://2009.igem.org/Team:IPN-UNAM-Mexico/Notebook/August_6_2009#August_6th"><img src="https://static.igem.org/mediawiki/2009/3/38/AnteriorMX.JPG" alt="Previous" width="70" height="70" align="left" border="0"></a> </td>
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                                    <p align="left">&nbsp;</p>
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                                    <p align="left">&nbsp;</p>
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                                    <p align="left">&nbsp;</p>
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                                    <p align="left">&nbsp;</p>                                  </td>
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Latest revision as of 08:15, 14 October 2009



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August 7th, 2009


Digestion Digestion using EcoRI :

  • plasmidicDNA..........3μl
  • Buffer 2....................1μl     x30
  • BSA..........................0.5μl  x30
  • EcoRI enzyme......... 0.5μl   x30
  • H2O..........................5μl
 

An electrophoresis gel was ran with the following quantities:

  • 3μl marker 1kb
  • Buffer 2 ................ 1μl.
  • 3μl DNA+ 3μl of colorant

All samples were digested with EcoRI in order to linearized them.

Line 1 1kb | 3.1 ....* 3.4|9.1....* 9.9|9.8.. 1|1kb|*18.2....18.4|19.1*....19.4|1kb

line  2 1kb|22.1.......*22.4|24.2”   16.2” 14.1 9.4|1kb

the ones marked with * are plasmids extracted today.

 

 

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