Team:UNIPV-Pavia/Methods Materials/Glycerol stock

From 2009.igem.org

(Difference between revisions)
(→Glycerol stocks)
 
(21 intermediate revisions not shown)
Line 1: Line 1:
-
{{UNIPV-Pavia/StyleCss}}
 
<html>
<html>
-
<head>
+
 
-
<link rel="stylesheet" type="text/css" href="http://bioinfo.unipv.it/igem/templates/SpryAssets/SpryMenuBar.js" title="Stile"/>
+
-
</head>
+
-
<body>
+
<table width="100%">
<table width="100%">
   <tr>
   <tr>
<td align="center">
<td align="center">
-
<img width="100%" height="180px" src="https://static.igem.org/mediawiki/2009/c/c2/UNIPV_title_MandM.jpg" ></img>
+
<img width="100%" height="180px" src="https://static.igem.org/mediawiki/2009/d/de/UNIPV_title_M%26M.jpg" ></img>
</td>
</td>
   </tr>
   </tr>
</table>
</table>
-
</body>
+
 
</html>
</html>
-
<table>
+
<table width="100%">
   <tr>
   <tr>
-
     <td valign="top" width= "23%">
+
     <td valign="top" width= "23%" rowspan="2">
{{UNIPV-Pavia/Menu}}
{{UNIPV-Pavia/Menu}}
     </td>
     </td>
-
     <td>
+
<html>
-
 
+
     <td width="50%"></td>
-
<h1>Protocols</h1>
+
    <td align="center"><br><bR>
-
 
+
    <font face="Pristina" size="50" ><b>Protocols</b></font>
-
*[[Team:UNIPV-Pavia/Methods_Materials/Lb|Lb medium]]
+
    </td>
-
*[[Team:UNIPV-Pavia/Methods_Materials/Transformation|Transformation]]
+
    <td width="50px"></td>
-
*[[Team:UNIPV-Pavia/Methods_Materials/XGal|X-Gal staining protocol for beta galactosidase (blue/white screening)]]
+
</html>
-
*[[Team:UNIPV-Pavia/Methods_Materials/Glycerol stock|Glycerol stock]]
+
  </tr>
-
*[[Team:UNIPV-Pavia/Methods_Materials/PlasmidExtraction|Plasmid extraction]]
+
<tr>
-
*[[Team:UNIPV-Pavia/Methods_Materials/Digestion|BioBrick digestion with restriction enzymes]]
+
  <td> {{UNIPV-Pavia/Protocols}}</td>
-
*[[Team:UNIPV-Pavia/Methods_Materials/GelExtraction|DNA gel extraction]]
+
</tr>
-
*[[Team:UNIPV-Pavia/Protocols/Precipitation|DNA precipitation with sodium acetate]]
+
</table>
-
*[[Team:UNIPV-Pavia/Methods_Materials/Ligation|Ligation]]
+
-
*[[Team:UNIPV-Pavia/Methods_Materials/Pcr|PCR]]
+
<br>
<br>
-
=Gycerol stock= <br>
+
== '''Glycerol stocks''' ==
-
''(estimated time: 15 min + freezing time)''''Italic text''
+
''(estimated time: 15 min + freezing time)''
<br>
<br>
<br>
<br>
-
'''Materials needed:'''
+
<table width='80%'><tr><td>
-
*'''sample of overnight incubated bacteria'''
+
*Mix 750 ul from the 5 ml sample of the incubated bacteria with 250 ul of 80% glycerol
-
*'''80% glycerol'''
+
-
<br>
+
-
*Mix 750 ul from the 5 ml sample of overnight incubated bacteria with 250 ul of 80% glycerol
+
*Leave at -20°C for one day
*Leave at -20°C for one day
*Move to -80°C the day after
*Move to -80°C the day after
-
    </td>
+
<br><br><br><br><br><br><br><br><br><br><br><br><br><br><br>
-
  </tr>
+
<br></td><td align='center' valign='top'  width='300px'>
-
</table>
+
 
 +
<table border='1'>
 +
<tr bgcolor='yellow'><td>INGREDIENTS:
 +
</td></tr>
 +
<tr><td>
 +
<font class='label'>
 +
'''o/n or in exp phase bacterial culture'''<hr color='white' width='70%'>
 +
'''80% glycerol'''</font>
 +
</td></tr></table><br><br><br><br>
 +
</td></tr></table>

Latest revision as of 10:27, 21 October 2009

EthanolPVanimation.gif



Protocols


Glycerol stocks

(estimated time: 15 min + freezing time)

  • Mix 750 ul from the 5 ml sample of the incubated bacteria with 250 ul of 80% glycerol
  • Leave at -20°C for one day
  • Move to -80°C the day after

















INGREDIENTS:

o/n or in exp phase bacterial culture

80% glycerol





Retrieved from "http://2009.igem.org/Team:UNIPV-Pavia/Methods_Materials/Glycerol_stock"