Team:UNIPV-Pavia/Methods Materials/Glycerol stock

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Latest revision as of 10:27, 21 October 2009

Protocols

Glycerol stocks

(estimated time: 15 min + freezing time)

Mix 750 ul from the 5 ml sample of the incubated bacteria with 250 ul of 80% glycerol
Leave at -20°C for one day
Move to -80°C the day after

INGREDIENTS:

o/n or in exp phase bacterial culture

80% glycerol

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-{{UNIPV-Pavia/Protocols}}
+<html>
+<table width="100%">
+  <tr>
+	<td align="center">
+	<img width="100%" height="180px" src="https://static.igem.org/mediawiki/2009/d/de/UNIPV_title_M%26M.jpg" ></img>
+	</td>
+  </tr>
+</table>
+</html>
+<table width="100%">
+  <tr>
+    <td valign="top" width= "23%" rowspan="2">
+{{UNIPV-Pavia/Menu}}
+    </td>
+<html>
+    <td width="50%"></td>
+    <td align="center"><br><bR>
+     <font face="Pristina" size="50" ><b>Protocols</b></font>
+    </td>
+    <td width="50px"></td>
+</html>
+  </tr>
+<tr>
+  <td> {{UNIPV-Pavia/Protocols}}</td>
+</tr>
+</table>
 <br>
-== Gycerol stock ==
+== '''Glycerol stocks''' ==
+''(estimated time: 15 min + freezing time)''
 <br>
-''(estimated time: 15 min + freezing time)''''Italic text''
 <br>
-<br>
+<table width='80%'><tr><td>
-'''Materials needed:'''
+*Mix 750 ul from the 5 ml sample of the incubated bacteria with 250 ul of 80% glycerol
-*'''sample of overnight incubated bacteria'''
-*'''80% glycerol'''
-<br>
-*Mix 750 ul from the 5 ml sample of overnight incubated bacteria with 250 ul of 80% glycerol
 *Leave at -20°C for one day
 *Move to -80°C the day after
-    </td>
+<br><br><br><br><br><br><br><br><br><br><br><br><br><br><br>
-  </tr>
+<br></td><td align='center' valign='top'  width='300px'>
-</table>
+<table border='1'>
+<tr bgcolor='yellow'><td>INGREDIENTS:
+</td></tr>
+<tr><td>
+<font class='label'>
+'''o/n or in exp phase bacterial culture'''<hr color='white' width='70%'>
+'''80% glycerol'''</font>
+</td></tr></table><br><br><br><br>
+</td></tr></table>