Team:NCTU Formosa/Notebook/Calendar

From 2009.igem.org

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</ul>
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<ul class="select">
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<li><a href="https://2009.igem.org/Team:NCTU_Formosa/Team" target="_self"><b>Team</b></a>
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    <ul class="select">
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    <li><a><b>Team</b></a>
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<div class="select_sub">
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<ul class="sub">
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<li><a href="https://2009.igem.org/Team:NCTU_Formosa/Team" target="_self">Team</a></li>
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                <li><a href="http://life.nctu.edu.tw/~cptlab/Picasa%20HTML%20Exports/2009_iGEM-NCTU_Formosa/20091013.html" target="_blank">Gallery</a></li>
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</ul>
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</div>
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<div class="select_sub">
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            <li><a href="https://2009.igem.org/Team:NCTU_Formosa/Project/Abstract" target="_self">Abstract</a></li>
<li><a href="https://2009.igem.org/Team:NCTU_Formosa/Project/Introduction" target="_self">Introduction</a></li>
<li><a href="https://2009.igem.org/Team:NCTU_Formosa/Project/Introduction" target="_self">Introduction</a></li>
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<li><a href="https://2009.igem.org/Team:NCTU_Formosa/Project/Components" target="_self">Components</a></li>
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<li><a href="https://2009.igem.org/Team:NCTU_Formosa/Project/Design" target="_self">Design</a></li>
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<li><a href="https://2009.igem.org/Team:NCTU_Formosa/Project/Construction" target="_self">Construction</a></li>
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                <li><a href="https://2009.igem.org/Team:NCTU_Formosa/Project/Parts" target="_self">Parts</a></li>
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<li><a href="https://2009.igem.org/Team:NCTU_Formosa/Project/Protocol" target="_self">Protocol</a></li>
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                <li><a href="https://2009.igem.org/Team:NCTU_Formosa/Project/Project_new" target="_self">New Idea</a></li>
<li><a href="https://2009.igem.org/Team:NCTU_Formosa/Project/Safety" target="_self">Safety</a></li>
<li><a href="https://2009.igem.org/Team:NCTU_Formosa/Project/Safety" target="_self">Safety</a></li>
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</ul>
</ul>
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<ul class="select">
<ul class="select">
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    <li><a><b>Notebook</b></a>
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<li><a><b>Results</b></a>
<div class="select_sub">
<div class="select_sub">
<ul class="sub">
<ul class="sub">
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<li><a href="https://2009.igem.org/Team:NCTU_Formosa/Notebook/Calendar" target="_self">Calendar</a></li>
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<li><a href="https://2009.igem.org/Team:NCTU_Formosa/WetLab/Labworks" target="_self">Lab works</a></li>
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<li><a href="https://2009.igem.org/Team:NCTU_Formosa/Notebook/Traveling" target="_self">Traveling</a></li>
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                <li><a href="https://2009.igem.org/Team:NCTU_Formosa/WetLab/Timer" target="_self">Timer</a></li>
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                <li><a href="https://2009.igem.org/Team:NCTU_Formosa/WetLab/Counter" target="_self">Counter</a></li>
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                <li><a href="https://2009.igem.org/Team:NCTU_Formosa/WetLab/Memory" target="_self">Memory</a></li>
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                <li><a href="https://2009.igem.org/Team:NCTU_Formosa/WetLab/Results_new" target="_self">New Idea</a></li>
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</ul>
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<ul class="select">
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<li><a><b>WetLab</b></a>
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    <li><a href="https://2009.igem.org/Team:NCTU_Formosa/Modeling" target="_self"><b>Modeling</b></a></li>
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</ul>
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<ul class="select">
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    <li><a><b>Notebook</b></a>
<div class="select_sub">
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<li><a href="https://2009.igem.org/Team:NCTU_Formosa/WetLab/Mainworks" target="_self">Mainworks</a></li>
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<li><a href="https://2009.igem.org/Team:NCTU_Formosa/Notebook/Calendar" target="_self">Calendar</a></li>
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<li><a href="https://2009.igem.org/Team:NCTU_Formosa/WetLab/Groupworks" target="_self">Groupworks</a></li>
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</ul>
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</li>
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<ul class="select">
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<li><a href="https://2009.igem.org/Team:NCTU_Formosa/Submitted Parts" target="_self"><b>Submitted Parts</b></a>
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<li><a href="https://2009.igem.org/Team:NCTU_Formosa/SubmittedParts" target="_self"><b>Submitted Parts</b></a>
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<div class="events"><p>It is the beginning today!!!!!!!! </p></div>
<div class="events"><p>It is the beginning today!!!!!!!! </p></div>
<div class="date"><p><a name="0717" id="7/17"></a> 7/17 </p></div>
<div class="date"><p><a name="0717" id="7/17"></a> 7/17 </p></div>
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<div class="events">
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<div class="events"><p>Lesson：Parts, Devices and Biobricks.&nbsp; In this lesson we learned about the principle of the construction of biobricks.</p></div>  
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  <p>Lesson：Parts, Devices and Biobricks.&nbsp; In this lesson we learned about the principle of the construction of biobricks.</p>
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<div class="date"><p><a name="0720" id="7/20"></a> 7/20~22 </p></div>
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  </div>  
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<div class="events"><p>To present 2008 IGEM projects.</p></div>
 +
<div class="date"><p><a name="0727" id="7/27"></a> 7/27~29 </p></div>
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<div class="events"><p>To present everyone’s primary idea.</p></div>  
</div>
</div>
</div>
</div>
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             <div class="date"><p><a name="0803" id="8/03"></a> 8/03 </p></div>
             <div class="date"><p><a name="0803" id="8/03"></a> 8/03 </p></div>
<div class="events"><p> A group duscussion of each one's personal idea.  </p></div>
<div class="events"><p> A group duscussion of each one's personal idea.  </p></div>
 +
            <div class="date"><p><a name="0805" id="8/05"></a> 8/05 </p></div>
 +
<div class="events"><p> To collate everyone’s advantages.</p></div>
                 <div class="date"><p><a name="0807" id="8/07"></a> 8/07 </p></div>
                 <div class="date"><p><a name="0807" id="8/07"></a> 8/07 </p></div>
<div class="events"><p> A group discussion.  &nbsp; We decided what kind of projects we're going to construct. </p></div>
<div class="events"><p> A group discussion.  &nbsp; We decided what kind of projects we're going to construct. </p></div>
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                 we have to contribute a part of the whole project. </p></div>
                 we have to contribute a part of the whole project. </p></div>
   <div class="date"><p><a name="0818" id="8/18"></a> 8/18 </p></div>
   <div class="date"><p><a name="0818" id="8/18"></a> 8/18 </p></div>
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<div class="events"><p> We transformed the bricks we need from the well. &nbsp; </p></div>
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<div class="events"><p> We transformed the bricks we need from the well.  </p></div> 
 +
<div class="date"><p><a name="0821" id="8/21"></a> 8/21 </p></div>
 +
<div class="events"><p>we check DNA by agarose gel, SDS-PAGE.</p></div>
 +
<div class="date"><p><a name="0822" id="8/22"></a> 8/22~23 </p></div>
 +
<div class="events"><p>To transfer E. coli to new LB broth, and incubate for 16hr, then Extract plasmid.</p></div>
 +
<div class="date">
 +
  <p><a name="0828" id="8/28"></a> 8/28 </p>
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</div>
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<div class="events"><p>we design a logo of team NCTU_FORMOSA</p></div>
</div>
</div>
</div>
</div>
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<div class="month"><p><a name="sep" id="sep"></a> <h2> September </h2></p></div>
<div class="month"><p><a name="sep" id="sep"></a> <h2> September </h2></p></div>
<div class="innertext">   
<div class="innertext">   
 +
<div class="date"><p><a name="0901" id="9/1"></a> 9/1 </p></div>
 +
<div class="events"><p>The experiment is not so smoothly, we sink into infinite loop in the same experiments.</p></div>
 +
<div class="date"><p><a name="0902" id="9/2"></a> 9/2 </p></div>
 +
<div class="events"><p>To digest the front part (8/23) and the behind part (8/26) of the biobrick, ligate, transform and run gel to check that if digestion is OK or not, then spread on the plate, and incubate for 20hr.</p></div>
 +
<div class="date"><p><a name="0907" id="9/7"></a> 9/7 </p></div>
 +
<div class="events"><p>We use another enzyme to digest to double check if the plasmid is right or not</p></div>
<div class="date"><p><a name="0910" id="9/10"></a> 9/10 </p></div>
<div class="date"><p><a name="0910" id="9/10"></a> 9/10 </p></div>
-
<div class="events"><p>Today we have a meeting that ... </p></div>
+
<div class="events"><p>Today we have a meeting that we discuss how to solve the problems.</p></div>
 +
<div class="date"><p><a name="0914" id="9/14"></a> 9/14~25 </p></div>
 +
<div class="events"><p> The plasmid's sequences are wrong and we repeat the digestion, ligation, and transform again.</p></div>
 +
<div class="date"><p><a name="0916" id="9/16"></a> 9/16 </p></div>
 +
<div class="events"><p>BBa_K188343 is sequenced today.</p></div>
 +
<div class="date"><p><a name="0928" id="9/28"></a> 9/28 </p></div>
 +
<div class="events"><p>Degenerated PCR, using BBa_K145279 as template</p></div>
 +
<div class="date"><p><a name="0929" id="9/29"></a> 9/29 </p></div>
 +
<div class="events"><p>Degenerated PCR</p></div>
</div>
</div>
</div>
</div>
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<div class="month"><p><a name="oct" id="oct"></a> <h2> October </h2></p></div>
<div class="month"><p><a name="oct" id="oct"></a> <h2> October </h2></p></div>
<div class="innertext">
<div class="innertext">
 +
            <div class="date"><p><a name="1002" id="10/2"></a> 10/2 </p></div>
 +
<div class="events"><p>Degenerated PCR. Restriction enzyme map.</p></div>
 +
            <div class="date"><p><a name="1007" id="10/7"></a> 10/7 </p></div>
 +
<div class="events"><p>Gel purification. To ligate gel purification products</p></div>
 +
            <div class="date"><p><a name="1011" id="10/11"></a> 10/11 </p></div>
 +
<div class="events"><p>to make restriction enzyme map. To select 14 plasmids</p></div>
             <div class="date"><p><a name="1012" id="10/12"></a> 10/12 </p></div>
             <div class="date"><p><a name="1012" id="10/12"></a> 10/12 </p></div>
-
<div class="events"><p> We had a team work to work on the wiki web.  </p></div>  
+
<div class="events"><p> We had a team work to work on the wiki web. Flow cytometer.  </p></div>  
             <div class="date"><p><a name="1014" id="10/14"></a> 10/14 </p></div>
             <div class="date"><p><a name="1014" id="10/14"></a> 10/14 </p></div>
-
<div class="events"><p> Submitted the parts. </p></div>  
+
<div class="events"><p> Submitted the parts to be sequenced.</p></div>  
             <div class="date"><p><a name="1018" id="10/18"></a> 10/18 </p></div>
             <div class="date"><p><a name="1018" id="10/18"></a> 10/18 </p></div>
<div class="events"><p> Updated the web of notebook and the project. &nbsp; We also had a group discussion about our results and the data.</p></div>
<div class="events"><p> Updated the web of notebook and the project. &nbsp; We also had a group discussion about our results and the data.</p></div>
 +
            <div class="date"><p><a name="1021" id="10/21"></a> 10/21 </p></div>
 +
<div class="events"><p></p>To discuss our final result.</div>
</div>
</div>
</div>
</div>

Latest revision as of 02:56, 22 October 2009

July

7/15

It is the beginning today!!!!!!!!

7/17

Lesson：Parts, Devices and Biobricks.  In this lesson we learned about the principle of the construction of biobricks.

7/20~22

To present 2008 IGEM projects.

7/27~29

To present everyone’s primary idea.

August

8/03

A group duscussion of each one's personal idea.

8/05

To collate everyone’s advantages.

8/07

A group discussion.   We decided what kind of projects we're going to construct.

8/12

A group discussion.  We discussed about the principle and some details of the project. We also designed the first step of the experimence and the protocols.

8/17

Today we devided our team into 5 subgroups and started the experimence.   In each subgroup, we have to contribute a part of the whole project.

8/18

We transformed the bricks we need from the well.

8/21

we check DNA by agarose gel, SDS-PAGE.

8/22~23

To transfer E. coli to new LB broth, and incubate for 16hr, then Extract plasmid.

8/28

we design a logo of team NCTU_FORMOSA

September

9/1

The experiment is not so smoothly, we sink into infinite loop in the same experiments.

9/2

To digest the front part (8/23) and the behind part (8/26) of the biobrick, ligate, transform and run gel to check that if digestion is OK or not, then spread on the plate, and incubate for 20hr.

9/7

We use another enzyme to digest to double check if the plasmid is right or not

9/10

Today we have a meeting that we discuss how to solve the problems.

9/14~25

The plasmid's sequences are wrong and we repeat the digestion, ligation, and transform again.

9/16

BBa_K188343 is sequenced today.

9/28

Degenerated PCR, using BBa_K145279 as template

9/29

Degenerated PCR

October

10/2

Degenerated PCR. Restriction enzyme map.

10/7

Gel purification. To ligate gel purification products

10/11

to make restriction enzyme map. To select 14 plasmids

10/12

We had a team work to work on the wiki web. Flow cytometer.

10/14

Submitted the parts to be sequenced.

10/18

Updated the web of notebook and the project.   We also had a group discussion about our results and the data.

10/21

To discuss our final result.

Retrieved from "http://2009.igem.org/Team:NCTU_Formosa/Notebook/Calendar"