"
Team:Rice/Notebook
From 2009.igem.org
(Prototype team page) |
m |
||
| (One intermediate revision not shown) | |||
| Line 1: | Line 1: | ||
| - | + | Eventually this will get formatted differently (better), but until then... | |
| + | |||
| + | New practices for this year: | ||
| + | |||
| + | In order to better preserve our 2009 parts library, whenever you use a new BioBrick, remove the 15uL of water that you resuspended with, transfer it to a PCR tube, and store in the iGEM 2009 box at -20. Also make sure to list whatever parts you pull on this notebook page. (And, of course, make glycerol stocks of '''every''' part that you pull from the registry. | ||
| + | |||
| + | 5/23/09 | ||
| + | |||
| + | Thomas | ||
| + | |||
| + | Pulled parts: | ||
| + | |||
| + | (Tail switching) | ||
| + | |||
| + | BBa_I719005 - T7 promoter | ||
| + | |||
| + | BBa_I719015 - T7 promoter test circuit with GFP | ||
| + | |||
| + | BBa_B0034 - Strong RBS | ||
| + | |||
| + | BBa_B0015 - commonly used strong forward terminator | ||
| + | |||
| + | BBa_B1006 - new, strong forward terminator | ||
| + | |||
| + | (Tet selection) | ||
| + | |||
| + | pSB4K5 - low copy Kan vector | ||
| + | |||
| + | (Part for Dr. Beason) | ||
| + | |||
| + | BBa_J45200 | ||
| + | |||
| + | from the 2009 library plates. | ||
| + | |||
| + | |||
| + | Used 1uL of each to HS transform into XL1 competent cells. | ||
Latest revision as of 07:13, 25 May 2009
Eventually this will get formatted differently (better), but until then...
New practices for this year:
In order to better preserve our 2009 parts library, whenever you use a new BioBrick, remove the 15uL of water that you resuspended with, transfer it to a PCR tube, and store in the iGEM 2009 box at -20. Also make sure to list whatever parts you pull on this notebook page. (And, of course, make glycerol stocks of every part that you pull from the registry.
5/23/09
Thomas
Pulled parts:
(Tail switching)
BBa_I719005 - T7 promoter
BBa_I719015 - T7 promoter test circuit with GFP
BBa_B0034 - Strong RBS
BBa_B0015 - commonly used strong forward terminator
BBa_B1006 - new, strong forward terminator
(Tet selection)
pSB4K5 - low copy Kan vector
(Part for Dr. Beason)
BBa_J45200
from the 2009 library plates.
Used 1uL of each to HS transform into XL1 competent cells.
