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Team:Team:Imperial College London/Wetlab/Protocols/Cellculture/CC11
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| - | Sequence and ligation independent cloning to join multiple pieces of DNA in a single step. The procedure also takes less time than standard ligation techniques. The cloning team did, however, experience limited success with this method. | + | Sequence and ligation independent cloning (SLIC) was undertaken to join multiple pieces of DNA in a single step. The procedure also takes less time than standard ligation techniques. The cloning team did, however, experience limited success with this method. |
| - | The In-Fusion Advantage PCR Cloning kit from Clontech was used. The protocol(in .pdf format) can be found [http://www.clontech.com/images/pt/PT4065-1.pdf here]. | + | The In-Fusion Advantage PCR Cloning kit from Clontech was used. The protocol (in .pdf format) can be found [http://www.clontech.com/images/pt/PT4065-1.pdf here]. |
{{Imperial/09/TemplateBottom}} | {{Imperial/09/TemplateBottom}} | ||
Latest revision as of 15:47, 18 October 2009
SLIC
Sequence and ligation independent cloning (SLIC) was undertaken to join multiple pieces of DNA in a single step. The procedure also takes less time than standard ligation techniques. The cloning team did, however, experience limited success with this method.
The In-Fusion Advantage PCR Cloning kit from Clontech was used. The protocol (in .pdf format) can be found [http://www.clontech.com/images/pt/PT4065-1.pdf here].
